MOLECULAR MEDICINE REPORTS 14: 2607-2613 2016
Angiopoietin-like protein 2 expression is suppressed by angiotensin II via the angiotensin II type 1 receptor in rat cardiomyocytes SHUYA WANG1,2, YING LI1, WEI MIAO1, HONG ZHAO1, FENG ZHANG1, NAN LIU1,2, GUOHAI SU1 and XIAOJUN CAI1,2 1
Department of Cardiovascular Medicine, Jinan Central Hospital Affiliated to Shandong University; 2 Shandong University School of Medicine, Jinan, Shandong 250013, P.R. China Received February 17, 2016; Accepted July 11, 2016 DOI: 10.3892/mmr.2016.5544
Abstract. The present study aimed to determine the inhibitory effects of angiotensin II (AngII) on angiopoietin‑like protein 2 (Angptl2) in rat primary cardiomyocytes, and to investigate the potential association between angiotensin II type 1 receptor (AT1R) and these effects. Cardiomyocytes were isolated from 3‑day‑old Wistar rats, and were cultured and identified. Subsequently, the expression levels of Angptl2 were detected following incubation with various concentrations of AngII for various durations using western blotting, reverse transcription‑quantitative polymerase chain reaction, enzyme‑linked immunosorbent assay and immunofluorescence. Finally, under the most appropriate conditions (100 nmol/l AngII, 24 h), the cardiomyocytes were divided into six groups: Normal, AngII, AngII + losartan, normal + losartan, AngII + PD123319 and normal + PD123319 groups, in order to investigate the possible function of AT1R in Angptl2 suppression. Losartan and PD123319 are antagonists of AT1R and angiotensin II type 2 receptor, respectively. The statistical significance of the results was analyzed using Student's t‑test or one‑way analysis of variance. The results demonstrated that Angptl2 expression was evidently suppressed (P
Angiopoietin-like protein 2 expression is suppressed by angiotensin II via the angiotensin II type 1 receptor in rat cardiomyocytes SHUYA WANG1,2, YING LI1, WEI MIAO1, HONG ZHAO1, FENG ZHANG1, NAN LIU1,2, GUOHAI SU1 and XIAOJUN CAI1,2 1
Department of Cardiovascular Medicine, Jinan Central Hospital Affiliated to Shandong University; 2 Shandong University School of Medicine, Jinan, Shandong 250013, P.R. China Received February 17, 2016; Accepted July 11, 2016 DOI: 10.3892/mmr.2016.5544
Abstract. The present study aimed to determine the inhibitory effects of angiotensin II (AngII) on angiopoietin‑like protein 2 (Angptl2) in rat primary cardiomyocytes, and to investigate the potential association between angiotensin II type 1 receptor (AT1R) and these effects. Cardiomyocytes were isolated from 3‑day‑old Wistar rats, and were cultured and identified. Subsequently, the expression levels of Angptl2 were detected following incubation with various concentrations of AngII for various durations using western blotting, reverse transcription‑quantitative polymerase chain reaction, enzyme‑linked immunosorbent assay and immunofluorescence. Finally, under the most appropriate conditions (100 nmol/l AngII, 24 h), the cardiomyocytes were divided into six groups: Normal, AngII, AngII + losartan, normal + losartan, AngII + PD123319 and normal + PD123319 groups, in order to investigate the possible function of AT1R in Angptl2 suppression. Losartan and PD123319 are antagonists of AT1R and angiotensin II type 2 receptor, respectively. The statistical significance of the results was analyzed using Student's t‑test or one‑way analysis of variance. The results demonstrated that Angptl2 expression was evidently suppressed (P
