EXPERIMENTAL AND THERAPEUTIC MEDICINE 14: 1722-1726, 2017
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Allicin improves carotid artery intima‑media thickness in coronary artery disease patients with hyperhomocysteinemia DE‑SHAN LIU1*, SHU‑LI WANG2*, JUN‑MEI LI3, ER‑SHUN LIANG1, MING‑ZHONG YAN3 and WEI GAO1 1
2
Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan, Shandong 250012; Department of Geriatrics, Linyi People's Hospital, Linyi, Shandong 276003; 3Department of Cardiovascular Medicine, Penglai Traditional Chinese Medicine Hospital of Shandong, Penglai, Shandong 265600, P.R. China Received August 30, 2015; Accepted January 26, 2017 DOI: 10.3892/etm.2017.4698
Abstract. Homocysteine (Hcy) is an important and independent risk factor for atherosclerotic diseases, such as coronary artery disease and ischemic cerebrovascular disease. Increased carotid artery intima‑media thickness (IMT) is a non‑invasive marker of systemic atherosclerosis. Allicin treatment may decrease serum Hcy levels and improve impaired endothelial function in rats with hyperhomocysteinemia (HHcy). The present study hypothesized that allicin has an anti‑atherosclerotic effect in coronary heart disease and tested the effects of allicin treatment on carotid artery IMT and plasma Hcy levels in coronary heart disease patients with HHcy. Sixty‑two coronary heart disease patients with HHcy were randomly divided into an allicin group and a control group. All patients underwent diagnostic assessment, plasma Hcy assay, blood lipid measurement and B‑mode ultrasound of the carotid artery prior to and after treatment. Plasma Hcy levels were determined by high‑performance liquid chromatography and fluorescence detection. Carotid artery IMT was calculated using an automated algorithm based on a validated edge‑detection technique. After 12 weeks, significant decreases in carotid artery IMT, plasma Hcy levels, total cholesterol and triglycerides were observed in the allicin group (all P1.0 mm.
Blood sampling. Blood samples were collected after overnight fasting. Serum was separated within 1 h and used for measuring of total cholesterol (TC) and triglyceride (TG). Hcy levels were determined in EDTA‑treated plasma.
Plasma Hcy levels. The Hcy levels are shown in Table II. The basal plasma Hcy concentration did not differ between the two groups. A significant decrease of Hcy was observed in the allicin group after 12 weeks (P
1722
Allicin improves carotid artery intima‑media thickness in coronary artery disease patients with hyperhomocysteinemia DE‑SHAN LIU1*, SHU‑LI WANG2*, JUN‑MEI LI3, ER‑SHUN LIANG1, MING‑ZHONG YAN3 and WEI GAO1 1
2
Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan, Shandong 250012; Department of Geriatrics, Linyi People's Hospital, Linyi, Shandong 276003; 3Department of Cardiovascular Medicine, Penglai Traditional Chinese Medicine Hospital of Shandong, Penglai, Shandong 265600, P.R. China Received August 30, 2015; Accepted January 26, 2017 DOI: 10.3892/etm.2017.4698
Abstract. Homocysteine (Hcy) is an important and independent risk factor for atherosclerotic diseases, such as coronary artery disease and ischemic cerebrovascular disease. Increased carotid artery intima‑media thickness (IMT) is a non‑invasive marker of systemic atherosclerosis. Allicin treatment may decrease serum Hcy levels and improve impaired endothelial function in rats with hyperhomocysteinemia (HHcy). The present study hypothesized that allicin has an anti‑atherosclerotic effect in coronary heart disease and tested the effects of allicin treatment on carotid artery IMT and plasma Hcy levels in coronary heart disease patients with HHcy. Sixty‑two coronary heart disease patients with HHcy were randomly divided into an allicin group and a control group. All patients underwent diagnostic assessment, plasma Hcy assay, blood lipid measurement and B‑mode ultrasound of the carotid artery prior to and after treatment. Plasma Hcy levels were determined by high‑performance liquid chromatography and fluorescence detection. Carotid artery IMT was calculated using an automated algorithm based on a validated edge‑detection technique. After 12 weeks, significant decreases in carotid artery IMT, plasma Hcy levels, total cholesterol and triglycerides were observed in the allicin group (all P1.0 mm.
Blood sampling. Blood samples were collected after overnight fasting. Serum was separated within 1 h and used for measuring of total cholesterol (TC) and triglyceride (TG). Hcy levels were determined in EDTA‑treated plasma.
Plasma Hcy levels. The Hcy levels are shown in Table II. The basal plasma Hcy concentration did not differ between the two groups. A significant decrease of Hcy was observed in the allicin group after 12 weeks (P
