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Ag-incorporated FHA Coating on Pure Mg: Degradation and in Vitro Antibacterial Properties Changli Zhao, Peng Hou, Jiahua Ni, Pei Han, Yimin Chai, and Xiaonong Zhang ACS Appl. Mater. Interfaces, Just Accepted Manuscript • DOI: 10.1021/acsami.5b10825 • Publication Date (Web): 08 Feb 2016 Downloaded from http://pubs.acs.org on February 10, 2016

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ACS Applied Materials & Interfaces

Ag-incorporated FHA Coating on Pure Mg: Degradation and in Vitro Antibacterial Properties

Changli Zhao1,#, Peng Hou2,#, Jiahua Ni1, Pei Han2,*, Yimin Chai2,* and Xiaonong Zhang1,* 1

State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering,

Shanghai Jiao Tong University, Shanghai 200240, China 2

The Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,

Shanghai 200233, China

#

*

These two authors contribute equally. Corresponding

authors.

Xiaonong

Zhang,

Tel/fax:

+86

21

34202759,

email:

[email protected]; Pei Han, Tel/fax: +86 21 64369181, email: [email protected]; Yimin Chai, Tel/fax: +86 21 64369181, email: [email protected].

Abstract

Fluoridated hydroxyapatite (FHA) coating can help retard the degradation of magnesium, and possess good biocompatibility. However, the antibacterial property of FHA is very limited. In this work, we aimed to incorporate silver into FHA structure to fabricate biocompatible and antibacterial coatings with enhanced anticorrosion property. The results showed that the Ag-FHA coating prepared by electrochemical deposition and subsequent immersion in AgNO3 solution was superior to the Ag-FHA coating prepared by co-electrodeposition in terms of crystal structure, 1 / 24

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surface morphology and corrosion resistance. The release of Ag+ ion causing high anti-planktonic bacterial rate and excellent anti-adherence property to MRSA. Meanwhile, good cell compatibility of MC3T3-E1 including cell viability, cell adhesion and cell morphology was achieved under the controlled degradation. The balance of degradation and antimicrobial property of Ag incorporated FHA coating made it an alternative in the application of surface modification for biodegradable Mg. Keywords: Magnesium, FHA coating, Silver, Degradation, Antibacterial properties

1. Introduction

As candidate implant biomaterials, magnesium and its alloys have been experienced intensive studies in the recent decade. Several pilot clinical researches have been launched. BIOTRONIK has developed drug eluting absorbable metal scaffolds (DREAMS) based on WE43 Mg alloy and the two-year clinical data (totally 44 patients) results reported no cardiac death and no scaffold thrombosis, and target lesion failure at two years was stable at 6.8%. A second, prospective, multi-centre clinical trial (BIOSOLVE-II) evaluating on the safety and efficacy of the latest generation DREAMS has just announced finishing enrolment (total of 122 patients) and expected performing 1, 6, 12, 24 and 36 months follow-up investigations. The MAGNEZIX bone screws made of MgYREZr have been proved not to cause foreign body reaction, osteolysis, or systemic inflammatory reaction and be equivalent to titanium screws in clinical mild hallux valgus deformities surgery.1 The MAGNEZIX screw has obtained CE mark for medical devices.

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ACS Applied Materials & Interfaces

Taken advantage of biodegradability, Mg implants can hydrolyse in body fluid environment when performing repair functions and avoid second removal, benefiting both patients and surgeons. However, the biodegradation rate is crucial for Mg to match the tissue repair. Unfortunately, few bare Mg and alloys meet the requirement, resulting in problems such as H2 accumulation, excessive local alkalization, etc.2 In this case, surface coatings which aim to construct a temporary surface possessing good corrosion resistance and biocompatibility are carried out.3 In our previous work, a fluoridated hydroxyapatite (Ca10(PO4)6(OH)2-xFx, 0

Ag-Incorporated FHA Coating on Pure Mg: Degradation and in Vitro Antibacterial Properties.

Fluoridated hydroxyapatite (FHA) coating can help retard the degradation of magnesium, and possess good biocompatibility. However, the antibacterial p...
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