Eur J Clin Microbiol Infect Dis DOI 10.1007/s10096-014-2137-4
ARTICLE
Aerobic culture of anaerobic bacteria using antioxidants: a preliminary report B. La Scola & S. Khelaifia & J.-C. Lagier & D. Raoult
Received: 16 January 2014 / Accepted: 23 April 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Antioxidants have been shown to help the growth of anaerobic bacteria. We were able to grow six anaerobe species (including Fusobacterium necrophorum and Ruminococcus gravus) and seven aerobic species all aerobically in Schaedler agar tubes and agar plates with high doses of ascorbic acid and/or glutathione. This may deeply change strategies for culturing bacteria.
identified using molecular techniques from human samples [9]. A critical problem in anaerobic culture is to avoid oxygen toxicity, which can be accomplished by depleting the atmosphere and culture medium of oxygen or by using antioxidant molecules. In this preliminary work, we evaluated the efficacy of the antioxidant molecules ascorbic acid and glutathione in allowing the cultivation of strict anaerobes and microaerophilic bacteria in the presence of oxygen.
Introduction Anaerobic bacteria are the most common microbes in the human microbiome, especially in the respiratory and the digestive tracts [1]. Moreover, they are critical in food-borne infections, brain abscesses and lung infections. However, cultivating these organisms is difficult given the technical requirements and oxygenation of samples during transport that may kill extremely oxygen-sensitive bacteria [2]. Finally, paradoxically fewer laboratories are equipped to cultivate anaerobic bacteria now than previously, despite current molecular techniques, and MALDI-TOF MS have simplified their identification [3–7]. Culturomics presents a clearer view of the bacteria associated with most humans, and it is estimated that approximately 2,000 bacterial species, many of which are anaerobic, have been isolated at least once in humans [8]. Therefore, we seek to optimize techniques for anaerobic cultures to improve documentation of multimicrobial infections that include anaerobes and to grow uncultivated bacteria that were B. La Scola : S. Khelaifia : J.
ARTICLE
Aerobic culture of anaerobic bacteria using antioxidants: a preliminary report B. La Scola & S. Khelaifia & J.-C. Lagier & D. Raoult
Received: 16 January 2014 / Accepted: 23 April 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Antioxidants have been shown to help the growth of anaerobic bacteria. We were able to grow six anaerobe species (including Fusobacterium necrophorum and Ruminococcus gravus) and seven aerobic species all aerobically in Schaedler agar tubes and agar plates with high doses of ascorbic acid and/or glutathione. This may deeply change strategies for culturing bacteria.
identified using molecular techniques from human samples [9]. A critical problem in anaerobic culture is to avoid oxygen toxicity, which can be accomplished by depleting the atmosphere and culture medium of oxygen or by using antioxidant molecules. In this preliminary work, we evaluated the efficacy of the antioxidant molecules ascorbic acid and glutathione in allowing the cultivation of strict anaerobes and microaerophilic bacteria in the presence of oxygen.
Introduction Anaerobic bacteria are the most common microbes in the human microbiome, especially in the respiratory and the digestive tracts [1]. Moreover, they are critical in food-borne infections, brain abscesses and lung infections. However, cultivating these organisms is difficult given the technical requirements and oxygenation of samples during transport that may kill extremely oxygen-sensitive bacteria [2]. Finally, paradoxically fewer laboratories are equipped to cultivate anaerobic bacteria now than previously, despite current molecular techniques, and MALDI-TOF MS have simplified their identification [3–7]. Culturomics presents a clearer view of the bacteria associated with most humans, and it is estimated that approximately 2,000 bacterial species, many of which are anaerobic, have been isolated at least once in humans [8]. Therefore, we seek to optimize techniques for anaerobic cultures to improve documentation of multimicrobial infections that include anaerobes and to grow uncultivated bacteria that were B. La Scola : S. Khelaifia : J.
