AIDS RESEARCH AND HUMAN RETROVIRUSES Volume 8, Number 8, 1992 Mary Ann Liebert, Inc., Publishers
Working Groups
Downloaded by Universitat de Barcelona CRAI from www.liebertpub.com at 01/10/19. For personal use only.
(See Section VIII)
VI Brief Definitive
Reports
Adjuvanticity of Stearyl Tyrosine on the Antibody Response to Peptide 503-535 from HIV GP160 CONSTANTIN BONA, ANN NIXON, RONALD KENNEDY, and HABIB ZAGHOUANI
WE ing
COMPARED the humoral immune response induced by immunization with an HIV-1 gpl60 peptide correspondto amino acid residues, 503-535, complexed with different adjuvants. Specifically, the antipeptide, anti-HIV-1 gpl60, and neutralizing antibody responses were measured in groups of mice and baboons that received peptide 503-535 conjugated to a carrier protein in either saline, alum, or stearyl tyrosine. The highest antibody responses were induced when mice and baboons were immunized with peptide complexed with stearyl tyrosine. Antipeptide antibodies bind to native gpl20 protein as assessed by radioimmunoprecipitation and exhibited neutraliz-
Department of Microbiology,
ing activity against HIV-1, IIIB isolate. These data indicate that stearyl tyrosine represents a potent candidate as a nontoxic adjuvant for HIV subunit or recombinant vaccines.
Mount Sinai School of Medicine, One Gustave
1377
Address
reprint requests
to:
Constantin Bono
Department of Microbiology Mount Sinai School of Medicine One Gustave Levy Place New York. NY 10029
Levy Place, New York NY
10029.
Working Groups
Downloaded by Universitat de Barcelona CRAI from www.liebertpub.com at 01/10/19. For personal use only.
(See Section VIII)
VI Brief Definitive
Reports
Adjuvanticity of Stearyl Tyrosine on the Antibody Response to Peptide 503-535 from HIV GP160 CONSTANTIN BONA, ANN NIXON, RONALD KENNEDY, and HABIB ZAGHOUANI
WE ing
COMPARED the humoral immune response induced by immunization with an HIV-1 gpl60 peptide correspondto amino acid residues, 503-535, complexed with different adjuvants. Specifically, the antipeptide, anti-HIV-1 gpl60, and neutralizing antibody responses were measured in groups of mice and baboons that received peptide 503-535 conjugated to a carrier protein in either saline, alum, or stearyl tyrosine. The highest antibody responses were induced when mice and baboons were immunized with peptide complexed with stearyl tyrosine. Antipeptide antibodies bind to native gpl20 protein as assessed by radioimmunoprecipitation and exhibited neutraliz-
Department of Microbiology,
ing activity against HIV-1, IIIB isolate. These data indicate that stearyl tyrosine represents a potent candidate as a nontoxic adjuvant for HIV subunit or recombinant vaccines.
Mount Sinai School of Medicine, One Gustave
1377
Address
reprint requests
to:
Constantin Bono
Department of Microbiology Mount Sinai School of Medicine One Gustave Levy Place New York. NY 10029
Levy Place, New York NY
10029.
