ADDITIONS AND CORRECTIONS VOLUME 291 (2016) PAGES 25462–25475 DOI 10.1074/jbc.A116.729202
Adaptor protein CD2AP and L-type lectin LMAN2 regulate exosome cargo protein trafficking through the Golgi complex. Sang-Ho Kwon, Sekyung Oh, Marisa Nacke, Keith E. Mostov, and Joshua H. Lipschutz
PAGES 25466 AND 25469:
Figs. 3D and 5C in the initially published version of this article were mistakenly repeated using the images from Figs. 3C and 5B, respectively. These figures have now been corrected with the original experimental images. The rat anti-HA antibody used in these figures was HRP-conjugated (anti-HA-HRP) and was added to the nitrocellulose membrane together with a rabbit anti--catenin antibody. The HA (GPRC5B-HA) and the -catenin chemiluminescent signals were therefore obtained from a single membrane per experiment. The ratio of GPRC5B-HA signal intensity in exosomes to that in total cell lysates from three independent experiments was calculated and normalized to the value calculated for the wild-type cells. This correction does not affect the results or conclusions of the work.
J. Biol. Chem. (2017) 292(40) 16523–16523 © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.
16523
Adaptor protein CD2AP and L-type lectin LMAN2 regulate exosome cargo protein trafficking through the Golgi complex. Sang-Ho Kwon, Sekyung Oh, Marisa Nacke, Keith E. Mostov, and Joshua H. Lipschutz
PAGES 25466 AND 25469:
Figs. 3D and 5C in the initially published version of this article were mistakenly repeated using the images from Figs. 3C and 5B, respectively. These figures have now been corrected with the original experimental images. The rat anti-HA antibody used in these figures was HRP-conjugated (anti-HA-HRP) and was added to the nitrocellulose membrane together with a rabbit anti--catenin antibody. The HA (GPRC5B-HA) and the -catenin chemiluminescent signals were therefore obtained from a single membrane per experiment. The ratio of GPRC5B-HA signal intensity in exosomes to that in total cell lysates from three independent experiments was calculated and normalized to the value calculated for the wild-type cells. This correction does not affect the results or conclusions of the work.
J. Biol. Chem. (2017) 292(40) 16523–16523 © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. Published in the U.S.A.
16523
