Tumor Biol. DOI 10.1007/s13277-014-1990-1
RESEARCH ARTICLE
ADAM17 is associated with EMMPRIN and predicts poor prognosis in patients with uterine cervical carcinoma Qin Xu & Mingang Ying & Guilin Chen & Ang Lin & Yunqing Xie & Noriyuki Ohara & Dongmei Zhou
Received: 18 December 2013 / Accepted: 17 April 2014 # International Society of Oncology and BioMarkers (ISOBM) 2014
Abstract Metalloproteinase activities of a disintegrin and metalloproteinase 17 (ADAM17), amphiregulin (AREG), extracellular matrix metalloproteinase inducer (EMMPRIN), and matrix metalloproteinases (MMPs) are involved in tumor biology. In patients with uterine cervical carcinoma, the expression and prognostic significance of ADAM17 remain to be fully elucidated. The expression of ADAM17, AREG, EMMPRIN, phospho-epidermal growth factor receptor (pEGFR), phospho-extracellular signal-regulated kinase (pERK), MMP-2, and MMP-9 was assessed by immunohistochemistry and/or Western blotting from cervical carcinoma cell lines, SiHa and HeLa cells, and cervical carcinoma tissues. AREG activity was measured by ELISA assay. The correlation of ADAM17, AREG, EMMPRIN, and MMP-9 expression with patients’ survival rates was assessed by Kaplan–Meier and Cox regression analyses. RNA interference (RNAi) experiment was performed using small interfering mRNA to ADAM17 and EMMPRIN. ADAM17, Q. Xu : G. Chen : A. Lin Department of Gynecology, Fujian Provincial Cancer Hospital, Fuzhou, China M. Ying (*) Department of Surgery, Fujian Provincial Cancer Hospital, 91 Maluding, Fuma Road, 350014 Fuzhou, Fujian, China e-mail: [email protected] Y. Xie Department of Research Center, Fujian Provincial Cancer Hospital, Fuzhou, China N. Ohara Department of Obstetrics and Gynecology, Sanda Municipal Hospital, Sanda, Japan D. Zhou Department of Pathology, Fujian Provincial Cancer Hospital, Fuzhou, China
EMMPRIN, and MMP-9 protein content was overexpressed in cervical carcinoma tissues compared with normal cervical tissues (P
RESEARCH ARTICLE
ADAM17 is associated with EMMPRIN and predicts poor prognosis in patients with uterine cervical carcinoma Qin Xu & Mingang Ying & Guilin Chen & Ang Lin & Yunqing Xie & Noriyuki Ohara & Dongmei Zhou
Received: 18 December 2013 / Accepted: 17 April 2014 # International Society of Oncology and BioMarkers (ISOBM) 2014
Abstract Metalloproteinase activities of a disintegrin and metalloproteinase 17 (ADAM17), amphiregulin (AREG), extracellular matrix metalloproteinase inducer (EMMPRIN), and matrix metalloproteinases (MMPs) are involved in tumor biology. In patients with uterine cervical carcinoma, the expression and prognostic significance of ADAM17 remain to be fully elucidated. The expression of ADAM17, AREG, EMMPRIN, phospho-epidermal growth factor receptor (pEGFR), phospho-extracellular signal-regulated kinase (pERK), MMP-2, and MMP-9 was assessed by immunohistochemistry and/or Western blotting from cervical carcinoma cell lines, SiHa and HeLa cells, and cervical carcinoma tissues. AREG activity was measured by ELISA assay. The correlation of ADAM17, AREG, EMMPRIN, and MMP-9 expression with patients’ survival rates was assessed by Kaplan–Meier and Cox regression analyses. RNA interference (RNAi) experiment was performed using small interfering mRNA to ADAM17 and EMMPRIN. ADAM17, Q. Xu : G. Chen : A. Lin Department of Gynecology, Fujian Provincial Cancer Hospital, Fuzhou, China M. Ying (*) Department of Surgery, Fujian Provincial Cancer Hospital, 91 Maluding, Fuma Road, 350014 Fuzhou, Fujian, China e-mail: [email protected] Y. Xie Department of Research Center, Fujian Provincial Cancer Hospital, Fuzhou, China N. Ohara Department of Obstetrics and Gynecology, Sanda Municipal Hospital, Sanda, Japan D. Zhou Department of Pathology, Fujian Provincial Cancer Hospital, Fuzhou, China
EMMPRIN, and MMP-9 protein content was overexpressed in cervical carcinoma tissues compared with normal cervical tissues (P
