RESEARCH ARTICLE
Acute rejection after kidney transplantation promotes graft fibrosis with elevated adenosine level in rat Mingliang Li1,2, Yingbo Dai1, Jun Lei1, Jin Tang1, Yihong Zhou1, Bing Xia1, Yang Xia3, Guangming Yin1*
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1 Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China, 2 Department of Urology, The First People’s Hospital of Xiangtan City, Xiangtan, China, 3 Department of Biochemistry and Molecular Biology, The University of Texas–Houston Medical School, Houston, Texas, United States of America * [email protected]
Abstract OPEN ACCESS
Aims
Citation: Li M, Dai Y, Lei J, Tang J, Zhou Y, Xia B, et al. (2017) Acute rejection after kidney transplantation promotes graft fibrosis with elevated adenosine level in rat. PLoS ONE 12(6): e0180211. https://doi.org/10.1371/journal. pone.0180211
Chronic allograft nephropathy is a worldwide issue with the major feature of progressive allograft fibrosis, eventually ending with graft loss. Adenosine has been demonstrated to play an important role in process of fibrosis. Our study aimed to investigate the relationship between adenosine and fibrosis in renal allograft acute rejection in rat.
Editor: Stanislaw Stepkowski, University of Toledo, UNITED STATES
Materials and methods
Received: April 10, 2017 Accepted: June 12, 2017 Published: June 26, 2017 Copyright: © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the Science and Technology Project of Hunan Province, China (NO. 2010FJ4088 ). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Wistar rats and SD rats were selected as experimental animals. Our study designed two groups. In the allograft transplantation group, kidneys of Wistar rats were orthotopically transplanted into SD rat recipients, the same species but not genetically identical, to induce acute rejection. Kidney transplantations of SD rats to SD rats which were genetically identical were served as the control. We established rat models and detected a series of indicators. All data were analyzed statistically. P
Acute rejection after kidney transplantation promotes graft fibrosis with elevated adenosine level in rat Mingliang Li1,2, Yingbo Dai1, Jun Lei1, Jin Tang1, Yihong Zhou1, Bing Xia1, Yang Xia3, Guangming Yin1*
a1111111111 a1111111111 a1111111111 a1111111111 a1111111111
1 Department of Urology, The Third Xiangya Hospital of Central South University, Changsha, Hunan, China, 2 Department of Urology, The First People’s Hospital of Xiangtan City, Xiangtan, China, 3 Department of Biochemistry and Molecular Biology, The University of Texas–Houston Medical School, Houston, Texas, United States of America * [email protected]
Abstract OPEN ACCESS
Aims
Citation: Li M, Dai Y, Lei J, Tang J, Zhou Y, Xia B, et al. (2017) Acute rejection after kidney transplantation promotes graft fibrosis with elevated adenosine level in rat. PLoS ONE 12(6): e0180211. https://doi.org/10.1371/journal. pone.0180211
Chronic allograft nephropathy is a worldwide issue with the major feature of progressive allograft fibrosis, eventually ending with graft loss. Adenosine has been demonstrated to play an important role in process of fibrosis. Our study aimed to investigate the relationship between adenosine and fibrosis in renal allograft acute rejection in rat.
Editor: Stanislaw Stepkowski, University of Toledo, UNITED STATES
Materials and methods
Received: April 10, 2017 Accepted: June 12, 2017 Published: June 26, 2017 Copyright: © 2017 Li et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: This work was supported by the Science and Technology Project of Hunan Province, China (NO. 2010FJ4088 ). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Wistar rats and SD rats were selected as experimental animals. Our study designed two groups. In the allograft transplantation group, kidneys of Wistar rats were orthotopically transplanted into SD rat recipients, the same species but not genetically identical, to induce acute rejection. Kidney transplantations of SD rats to SD rats which were genetically identical were served as the control. We established rat models and detected a series of indicators. All data were analyzed statistically. P
