Accepted Manuscript + Accumulation of CD208 mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients Yan-Hong Ni , PhD Xiao-Feng Huang , MD, PhD Liang Ding , MSC Zhi-Yong Wang , MD, PhD Qin-Gang Hu , MD, PhD Ya-Yi Hou , PhD PII:
S0278-2391(14)00564-3
DOI:
10.1016/j.joms.2014.04.038
Reference:
YJOMS 56335
To appear in:
Journal of Oral and Maxillofacial Surgery
Received Date: 20 March 2014 Revised Date:
13 April 2014
Accepted Date: 21 April 2014
Please cite this article as: Ni Y-H, Huang X-F, Ding L, Wang Z-Y, Hu Q-G, Hou Y-Y, Accumulation of + CD208 mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients, Journal of Oral and Maxillofacial Surgery (2014), doi: 10.1016/j.joms.2014.04.038. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Title page Accumulation of CD208+ mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients
Wang, MD, PhD2, Qin-Gang Hu, MD, PhD 2*, Ya-Yi Hou, PhD1*
RI PT
Yan-Hong, Ni PhD1,a , Xiao-Feng Huang, MD, PhD2,a, Liang Ding, MSC1, Zhi-Yong
1 Central Laboratory of Stomatology, Hospital of Stomatology and The State Key
SC
Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, People's Republic of China
M AN U
2 Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, P. R. China a
*
Corresponding author.
Qin-Gang Hu, PhD, MD,
TE D
These authors contributed equally to this work.
EP
Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, 30 Zhong Yang
AC C
Road, Nanjing 210008, P. R. China Tel.: +86 025 83620101 Fax: +86 025 83357174. E-mail: [email protected]
Yayi Hou, PhD
ACCEPTED MANUSCRIPT Medical School Nanjing University, PR China, 210093
RI PT
E-mail: yayihou@nju,edu.cn
AC C
EP
TE D
M AN U
SC
Tel.: +86-25-83686441; Fax: +86-25-83686441.
ACCEPTED MANUSCRIPT Accumulation of CD208+ mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients Abstract PURPOSE: We sought to investigate the distribution and clinical outcomes of
RI PT
tumor-infiltrating CD208+ mature dendritic cells (mDCs) in OSCC patients.
MATERIALS AND METHODS: Using immunohistochemical analysis, the
SC
distribution of CD208+ mDCs in adjacent non-neoplastic tissues (NT) and tumor tissue including tumor stroma (TS) and tumor nest (TN) of 79 OSCC patients were evaluated.
M AN U
The analysis was quantitative, and the number of positive cells was counted in 5 microscopic high-power fields (400×). At gene expression level, CD208 expression was also assessed by q-PCR. Kaplan Meier analyses were used to analyze the prognostic value of tumor infiltrating CD208+ mDCs.
TE D
RESULTS: The number of tumor infiltrating CD208+ mDCs was higher in TS and TN than in NT (P
S0278-2391(14)00564-3
DOI:
10.1016/j.joms.2014.04.038
Reference:
YJOMS 56335
To appear in:
Journal of Oral and Maxillofacial Surgery
Received Date: 20 March 2014 Revised Date:
13 April 2014
Accepted Date: 21 April 2014
Please cite this article as: Ni Y-H, Huang X-F, Ding L, Wang Z-Y, Hu Q-G, Hou Y-Y, Accumulation of + CD208 mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients, Journal of Oral and Maxillofacial Surgery (2014), doi: 10.1016/j.joms.2014.04.038. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT Title page Accumulation of CD208+ mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients
Wang, MD, PhD2, Qin-Gang Hu, MD, PhD 2*, Ya-Yi Hou, PhD1*
RI PT
Yan-Hong, Ni PhD1,a , Xiao-Feng Huang, MD, PhD2,a, Liang Ding, MSC1, Zhi-Yong
1 Central Laboratory of Stomatology, Hospital of Stomatology and The State Key
SC
Laboratory of Pharmaceutical Biotechnology, Division of Immunology, Medical School, Nanjing University, Nanjing, People's Republic of China
M AN U
2 Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, P. R. China a
*
Corresponding author.
Qin-Gang Hu, PhD, MD,
TE D
These authors contributed equally to this work.
EP
Department of Oral and Maxillofacial Surgery, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing Stomatological Hospital, 30 Zhong Yang
AC C
Road, Nanjing 210008, P. R. China Tel.: +86 025 83620101 Fax: +86 025 83357174. E-mail: [email protected]
Yayi Hou, PhD
ACCEPTED MANUSCRIPT Medical School Nanjing University, PR China, 210093
RI PT
E-mail: yayihou@nju,edu.cn
AC C
EP
TE D
M AN U
SC
Tel.: +86-25-83686441; Fax: +86-25-83686441.
ACCEPTED MANUSCRIPT Accumulation of CD208+ mature dendritic cells does not correlate with survival time in oral squamous cell carcinoma patients Abstract PURPOSE: We sought to investigate the distribution and clinical outcomes of
RI PT
tumor-infiltrating CD208+ mature dendritic cells (mDCs) in OSCC patients.
MATERIALS AND METHODS: Using immunohistochemical analysis, the
SC
distribution of CD208+ mDCs in adjacent non-neoplastic tissues (NT) and tumor tissue including tumor stroma (TS) and tumor nest (TN) of 79 OSCC patients were evaluated.
M AN U
The analysis was quantitative, and the number of positive cells was counted in 5 microscopic high-power fields (400×). At gene expression level, CD208 expression was also assessed by q-PCR. Kaplan Meier analyses were used to analyze the prognostic value of tumor infiltrating CD208+ mDCs.
TE D
RESULTS: The number of tumor infiltrating CD208+ mDCs was higher in TS and TN than in NT (P
