Plastic and Reconstructive Surgery • March 2014 Supplement

Sunday, March 9, 2014 149 Effect of Low Energy Laser on Skin Flap Survival: A Study in Murine Dorsal Skin Flap Model Dhaval Bhavsar, MBBS1; Mayer Tenenhaus, MD FACS2 1 University of Kansas Medical Center, Kansas City, KS, 2University of California San Diego, School of Medicine, San Diego, CA Introduction: Random pattern skin flaps are commonly employed in reconstructive surgery. Despite general dimensional design guidelines, distal flap necrosis occurs with some regularity. The application of Low energy LASER (LEL) at 635 nm has been shown to augment mitochondrial activity and ATP production. We studied effects of LEL on tissue perfusion, tissue survival and cytokine response in a murine skin flap model. Materials and Methods: A caudally based skin flap, 1.5 cm x 4 cm was designed over the dorsum of mice (n=28). Study group (n=14) received 635 nm Laser irradiation, 2 min. every day for up to 4 days, beginning immediately after surgery. Control group (n=14) did not receive LEL treatment. All other care was identical. Six animals in each group were sacrificed 24 hrs after surgery to obtain tissue samples for TNF-alpha. Laser Doppler measurements were performed immediately before and after surgery and on post-operative days 1, 4, and 7 for rest of the 8 animals in both groups. These animals were euthanized on day 7 and digital photos of flaps were taken. Flap necrosis was calculated with image analysis software. Six animals received anesthesia but did undergo surgery and served as SHAM subjects for skin biopsy for TNFalpha levels. Results: The mean flap loss in control and study animals was 54.6% and 23.7% respectively (P

Abstract 149: effect of low energy laser on skin flap survival: a study in murine dorsal skin flap model.

Abstract 149: effect of low energy laser on skin flap survival: a study in murine dorsal skin flap model. - PDF Download Free
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