CASE REPORT ABORTION ASSOCIATED WITH HEMOPHILUS SOMNUS INFECTION IN A BOVINE FETUS A. A. VAN DREUMEL AND M. KIERSTEAD*
Hemophilus somnus is commonly associated with septicemia and secondary thromboembolic meningoencephalitis (TEME) in feedlot cattle (4, 6). The disease was first reported in Canada in 1970 (6). Since that time TEME has become one of the most serious infectious diseases of feedlot cattle in Ontario. More recently H. somnus has been associated with urogenital tract infections in cows and birth of weak calves (7). The purpose of this report is to record the isolation of H. somnus from an aborted bovine fetus and the lesions which were observed in the fetus and placenta. History A seven and one-half month old Limousin cross fetus and placenta were submitted for necropsy. There had been two abortions in the 75 cow herd over a period of three weeks. The first fetus was aborted at approximately seven and one-half months and the second at nine months of gestation. The cow which aborted at seven and onehalf months retained her after-birth. This dam was bred naturally, the dam which aborted at nine months was bred artificially. There was no recent history of illness in the herd. The cows were not vaccinated for infectious bovine rhinotracheitis (IBR). The ration consisted of good quality mixed hay and mineral supplement. The cows were kept in loose housing with access to a yard.
FIGuRE 1. Placentitis and fibrinoid necrosis of placental vessels (intercotyledonary area). x50.
_
:
&
:
:.
:~~'
FiGuRE 2. Fibrinoid thrombus and medial necrosis of artery in placenta x 2.
stained sections. Lung stomach content and Gross Lesions The fetus was well preserved. The peritoneal placenta were collected for microbiological cavity contained clotted blood due to lacera- examination. tions of the liver capsule. The cotyledons were necrotic and edematous. Some areas of inter- Microscopic Lesions Sections of placenta revealed marked edema cotyledonary placenta were hemorrhagic and of the stroma. Focal areas of the stroma were necrotic. Portions of placenta, lung, brain, liver, kid- moderately infiltrated by mononuclear cells, e eton.Ln,soac otnneutron macrophages with a few ney, myocardium, spleen and intestine were 367predominantly routinely processed for hematoxylin and eosin phils (Figure 1). There was extensive fibrinoid (H & E.) and Brown and Brenn (Gram) necrosis of the media of large and mediumsized arteries. Many arteries had fibrinoid *Veterinary Services Laboratory, Ministry of thrombi in the lumina (Figure 2). A mild Agriculture and Food, Box 3612, Guelph, Ontario mononuclear cell reaction was evident in the media of some arteries (arteritis). There was NlH 6R8. CAN. VET. JOUR., vol. 16, no. 12, December, 1975
CANADIAN VETERINARY JOURNAL
DU,
FIGURE 3. Interstitial myocarditis in fetus associated with H. somnus infection. x5O.
FIGURE 5. Thrombus in meningeal vein of cerebral hemisphere. x5O.
FIGURE 4. Leukocytosis of capillaries in cerebellar peduncle. X50.
FiGuRE 6. Leukocytosis of bronchiolar artery with perivascular hemorrhage. Focal aggregate of lymphocytes adjacent to bronchiole. x20.
marked necrosis of the villi. Colonies of Gram positive cocci and small pleomorphic Gram negative bacilli were evident in the necrotic debris. Focal areas of interstitial myocarditis were evident in sections of myocardium (Figure 3). The inflammatory cells consisted predominantly of mononuclear cells. These lesions were most obvious in subepi- and endocardial areas. In addition, foci of marked cytoplasmic vacuolation of myocardial fibres (myocytolysis) were also evident. A few veins were stuffed with mononuclear cells which were mixed with fibrinous thrombi. Vascular oriented lesions were also present in sections of cerebral hemispheres, cerebellum, pons and medulla oblongata. Some of the meningeal and parenchymal vessels were stuffed with mononuclear cells (Figures 4 and 5). Many vessels had hyaline and fibrinoid thrombi (Figure 5) and there was marked perivascular edema. The endothelial cells in the affected vessels were hypertrophic. In the lung the alveolar septae were thickened due to a mononuclear cell inflammatory
cell reaction. Hyaline and fibrinoid thrombi were evident in many alveolar capillaries. Focal areas with hemorrhage and fibrin deposition were evident in alveolar and interlobular spaces. A mild mononuclear inflammatory cell reaction was frequently associated with the hemorrhages. Several medium-sized arteries were stuffed with mononuclear cells (Figure 6). Sections of kidney revealed hyaline thrombi in glomerular capillaries and focal areas of tubular necrosis in the cortex. Sections of liver showed a mild periportal mononuclear cell reaction. Fibrinoid thrombi and colonies of bacilli were evident in the sinusoids of the splenic red pulp.
Bacteriology Wet mounts in KOH of stomach content and cotyledon were negative for fungi. Direct smears stained by a modified Kosters (5) method were negative for Brucella. A gramstained smear of the stomach content showed no visible organisms, however, smears of the cotyledon showed large numbers of small Gram negative bacilli.
368
HEMOPHILUS SOMNUS
Specimens were cultured aseptically on blood agar plates, incubated in a moist atmosphere of 10% carbon dioxide, and MacConkey agar. At 24 hours incubation the only growth was that of an alpha hemolytic streptococcus. After 48 to 72 hours incubation, large numbers of small, shiny, umbonate colonies were isolated from the cotyledon and a few colonies from both the lung and stomach content. Smears of the colonies showed small pleomorphic Gram negative bacilli. When the colonies were massed with a loop, a definite lemon yellow colour was observed and a greenish hemolysis of the blood agar. Subcultures to MacConkey and nutrient agar showed no growth. A very light growth was obtained on an aerobic blood agar subculture after 72 hours incubation. The isolate exhibited no satelitic growth with Staph. aureus. It was non-motile, catalase-negative and oxidasepositive. Further tests were carried out in media containing 5% calfs serum, 5% ovine serum and 0.5% yeast extract. Urea, citrate, gelatin and M.R.-V.P. tests were negative. Indole was produced and nitrates reduced to nitrites. Glucose, mannose, maltose, xylose, trehalose, mannitol and sorbitol were fermented without gas production. Lactose, sucrose, dulcitol, arabinose, inositol, salicin, rhamnose, raffinose and galactose were not fermented. Cultural and biochemical characteristics indicate this organism to be identical to the Hemophilus-like organism isolated from cases of TEME. The micro-organism has been referred to as Hemophilus somnus (1).
Waldham et al (7) recently associated H. somnus with the birth of weak calves. These authors suggested that it may be a congenital infection in such calves since they frequently showed clinical signs at birth. We can only speculate with regard to the route of infection. We have isolated H. somnus
from purulent vaginal discharges of repeatbreeder cows. Direct invasion through the cervix is only one possible route of infection. Since the organism may cause a septicemia in adult animals, hematogenous infection of the placenta should also be considered. The significance of H. somnus in reproductive problems of cattle is not clear at this time. It is known that cows may carry the organism in their reproductive tracts. There is also a report of venereal transmission by an infected "marker" bull to virgin heifers (G. G. Klavano, personal communication). This observation could have serious implications in those herds where natural breeding or "marker" bulls are used. Serological surveys have also shown that the infection is widespread in areas where TEME occurs (3, 4). A more diligent search for H. somnus in aborted fetuses and placentas may raise the percentage of diagnoses due to infectious agents. Routine procedures in the examination of aborted fetuses, should include culturing of placentas and fetal stomach contents under 10% CO2 for at least 72 hours, particularly if Gram stains of the placenta revealed small Gram negative bacilli.
Summary H. somnus was isolated from the placenta, stomach contents and lung of seven and onehalf month old bovine fetus. The lesions in the Discussion fetus were characterized by vascular necrosis, The isolation of H. somnus from the placenta, thrombosis and leukocytosis in the brain, lung, fetal stomach contents and lung with septicemic myocardium and kidney. In addition, interstitial lesions in the placenta and several fetal organs pneumonia and myocarditis were prominent. strongly suggest that the abortion was due to The placental lesions consisted of thrombosis this organism. The vascular necrosis and vaseu- and medial necrosis of stromal arteries with litis with thrombosis in the placenta and fetal edema and placentitis. This appears to be the organs are similar to lesions associated with first recorded isolation of H. somnus from an H. somnus in older animals. aborted bovine fetus which showed septicemic All biochemical and sensitivity results of this lesions. isolate were similar to those of other H. somnus strains from a variety of bovine sources (G.A. Resume Garcia-Delgado, personal communication 1975). Les auteurs ont isole Hemophilus somnus du To our knowledge this is the first recorded iso- placenta, du contenu stomacal et des poumons lation of this organism from an aborted bovine d'un avorton bovin de sept mois et demi. Les fetus. Firehammer (2) reported the isolation of lesions de cet avorton se caracterisaient par de a Hemophilus organism from an aborted fetus la neerose vasculaire, de la thrombose et une and the dam's vaginal tract. The species was leucocytose du cerveau, des poumons, du myonot definitely identified and the biochemical carde et des reins; elles comprenaient aussi une characteristics reported did not correspond with pneumonie interstitielle et une myocardite. Le those of H. somnus. placenta presentait de la thrombose et de la 369
CANADIAN VETERINARY JOURNAL
n6crose de la media des arteres du stroma, ainsi que de roedeme et une placentite. II semble que ce soit la premiere fois qu'on rapporte l'isolation de H. somnus d'un avorton bovin presentant des lesions septicemiques. Addendum Since the preparation of this paper a case of bovine abortion associated with H. somnus was reported by D. W. Chladek. Am. J. vet. Res. 36: 1041. 1975. REFERENCES
1. BAILIE, W. E. Characterization of Hemophilus somnus (new species), a micro-organism isolated from infectious thrombo-embolic meningo-encephalomyelitis of cattle. Ph.D. Thesis. Kansas State University. 1969. 2. FnREAMMER, B. D. Bovine abortion due to Hemophilus species. J. Am. vet. med. Ass. 135:
421-422. 1959.
3. DmRKs, R. E., S. A. HANNA and R. C. DILL-
MAN. Epizootiology and pathogenesis of Hemophilus somnus infection. J. Am. vet. med. Ass. 163: 866-869. 1973. 4. KENNEDY, P. C., E. L. BIBERsEIN, J. A. HowARTH, L. M. FRAzIm and D. L. DUNGWORTH. Infectious meningo-encephalitis in cattle caused by a Hemophilus-like organism. Am. J. vet. Res. 21: 403-409. 1960. 5. SOLTYS, M. A. Bacteria and Fungi Pathogenic to Man and Animals. p. 498. London: Bailliere Tindall and Cox. 1963. 6. VAN DIREUMEL, A. A., R. A. CuRiTS and H. L. RUHNRE. Infectious thromboembolic meningo-encephalitis in Ontario feedlot cattle. Can. vet. J. 11: 125-130. 1970. 7. WALDHAM, D. G., R. F. HALL, W. A. MEINERSHAGEN, C. S. CARD and F. W. FRANK. Hemophilus somnus infection in the cow as a possible contributing factor to weak calf syndrome: Iso-
lation and animal inoculation studies. Am. J. vet. Res. 35: 1401-1403. 1974.
PUBLICIT9 CONCERNANT LES DROGUES D'APPLICATION
VETERINARY DRUG ADVERTISING
VgTgRINAIRE
The Canadian Veterinary Medical Association Drug Committee has advised the Bureau of Veterinary Medicine, Health Protection Branch of complaints received from veterinarians in connection with advertisements for veterinary drugs appearing in various Canadian publications or in literature distributed through the mails. Section 9 (1) of the Food and Drugs Act expressly prohibits the labelling, sale or advertising of "any drug in a manner that is false, misleading or deceptive or is likely to create an erroneous impression regarding its character, value, quantity, composition, merit or safety." Veterinarians who encounter advertisements or brochures for drugs intended for use in animals that they consider are a contravention of the Food and Drugs Act should contact: Bureau of Drug Surveillance Drugs Directorate Health Protection Branch Department of National Healh and Welfare Tunney's Pasture Ottawa, Ontario KlA 0L2 It will facilitate the work of the Branch if a copy of the advertisement, a reference to its source and a brief outline of the reasons for believing that the advertisement is deceptive are included in the communication.
Le Comite sur les medicaments de I'Association canadienne des Veterinaires a informe la Direction generale de la protection de la sante sur les drogues d'application veterinaire des plaintes formulees par les veterinaires au suiet d'annonces publicitaires parues dans diverses publications canadiennes ou dans des imprimes envoyes par courrier. L'article 9 (1) de la Loi des aliments et drogues interdit formellement 1'etiquetage, la vente ou la publicite de "quelque drogue de maniere fausse, trompeuse, ou mensongere, ou qui peut creer une fausse impression quant A la nature, valeur, quantite, composition, ou quant aux avantages ou a la surete de la drogue". Les veterinaires estimant qu'une reclame ou brochure concernant les drogues destinees aux animaux constitue une infraction a la Loi des aliments et drogues devraient s'adresser au: Bureau de la surveillance des m6dicaments Direction des drogues Direction g6n6rale de la protection de la santA MinistAre de la Sant6 et du Bien-;t.e social Parc Tunney Ottawa (Ontario) KIA 0L2 Pour faciliter le travail de la Direction, joindre a la lettre une copie de la r6clame, en indiquer la source et exposer brievement les raisons portant a croire qu'elle est mensongere.
370
Hemophilus somnus is commonly associated with septicemia and secondary thromboembolic meningoencephalitis (TEME) in feedlot cattle (4, 6). The disease was first reported in Canada in 1970 (6). Since that time TEME has become one of the most serious infectious diseases of feedlot cattle in Ontario. More recently H. somnus has been associated with urogenital tract infections in cows and birth of weak calves (7). The purpose of this report is to record the isolation of H. somnus from an aborted bovine fetus and the lesions which were observed in the fetus and placenta. History A seven and one-half month old Limousin cross fetus and placenta were submitted for necropsy. There had been two abortions in the 75 cow herd over a period of three weeks. The first fetus was aborted at approximately seven and one-half months and the second at nine months of gestation. The cow which aborted at seven and onehalf months retained her after-birth. This dam was bred naturally, the dam which aborted at nine months was bred artificially. There was no recent history of illness in the herd. The cows were not vaccinated for infectious bovine rhinotracheitis (IBR). The ration consisted of good quality mixed hay and mineral supplement. The cows were kept in loose housing with access to a yard.
FIGuRE 1. Placentitis and fibrinoid necrosis of placental vessels (intercotyledonary area). x50.
_
:
&
:
:.
:~~'
FiGuRE 2. Fibrinoid thrombus and medial necrosis of artery in placenta x 2.
stained sections. Lung stomach content and Gross Lesions The fetus was well preserved. The peritoneal placenta were collected for microbiological cavity contained clotted blood due to lacera- examination. tions of the liver capsule. The cotyledons were necrotic and edematous. Some areas of inter- Microscopic Lesions Sections of placenta revealed marked edema cotyledonary placenta were hemorrhagic and of the stroma. Focal areas of the stroma were necrotic. Portions of placenta, lung, brain, liver, kid- moderately infiltrated by mononuclear cells, e eton.Ln,soac otnneutron macrophages with a few ney, myocardium, spleen and intestine were 367predominantly routinely processed for hematoxylin and eosin phils (Figure 1). There was extensive fibrinoid (H & E.) and Brown and Brenn (Gram) necrosis of the media of large and mediumsized arteries. Many arteries had fibrinoid *Veterinary Services Laboratory, Ministry of thrombi in the lumina (Figure 2). A mild Agriculture and Food, Box 3612, Guelph, Ontario mononuclear cell reaction was evident in the media of some arteries (arteritis). There was NlH 6R8. CAN. VET. JOUR., vol. 16, no. 12, December, 1975
CANADIAN VETERINARY JOURNAL
DU,
FIGURE 3. Interstitial myocarditis in fetus associated with H. somnus infection. x5O.
FIGURE 5. Thrombus in meningeal vein of cerebral hemisphere. x5O.
FIGURE 4. Leukocytosis of capillaries in cerebellar peduncle. X50.
FiGuRE 6. Leukocytosis of bronchiolar artery with perivascular hemorrhage. Focal aggregate of lymphocytes adjacent to bronchiole. x20.
marked necrosis of the villi. Colonies of Gram positive cocci and small pleomorphic Gram negative bacilli were evident in the necrotic debris. Focal areas of interstitial myocarditis were evident in sections of myocardium (Figure 3). The inflammatory cells consisted predominantly of mononuclear cells. These lesions were most obvious in subepi- and endocardial areas. In addition, foci of marked cytoplasmic vacuolation of myocardial fibres (myocytolysis) were also evident. A few veins were stuffed with mononuclear cells which were mixed with fibrinous thrombi. Vascular oriented lesions were also present in sections of cerebral hemispheres, cerebellum, pons and medulla oblongata. Some of the meningeal and parenchymal vessels were stuffed with mononuclear cells (Figures 4 and 5). Many vessels had hyaline and fibrinoid thrombi (Figure 5) and there was marked perivascular edema. The endothelial cells in the affected vessels were hypertrophic. In the lung the alveolar septae were thickened due to a mononuclear cell inflammatory
cell reaction. Hyaline and fibrinoid thrombi were evident in many alveolar capillaries. Focal areas with hemorrhage and fibrin deposition were evident in alveolar and interlobular spaces. A mild mononuclear inflammatory cell reaction was frequently associated with the hemorrhages. Several medium-sized arteries were stuffed with mononuclear cells (Figure 6). Sections of kidney revealed hyaline thrombi in glomerular capillaries and focal areas of tubular necrosis in the cortex. Sections of liver showed a mild periportal mononuclear cell reaction. Fibrinoid thrombi and colonies of bacilli were evident in the sinusoids of the splenic red pulp.
Bacteriology Wet mounts in KOH of stomach content and cotyledon were negative for fungi. Direct smears stained by a modified Kosters (5) method were negative for Brucella. A gramstained smear of the stomach content showed no visible organisms, however, smears of the cotyledon showed large numbers of small Gram negative bacilli.
368
HEMOPHILUS SOMNUS
Specimens were cultured aseptically on blood agar plates, incubated in a moist atmosphere of 10% carbon dioxide, and MacConkey agar. At 24 hours incubation the only growth was that of an alpha hemolytic streptococcus. After 48 to 72 hours incubation, large numbers of small, shiny, umbonate colonies were isolated from the cotyledon and a few colonies from both the lung and stomach content. Smears of the colonies showed small pleomorphic Gram negative bacilli. When the colonies were massed with a loop, a definite lemon yellow colour was observed and a greenish hemolysis of the blood agar. Subcultures to MacConkey and nutrient agar showed no growth. A very light growth was obtained on an aerobic blood agar subculture after 72 hours incubation. The isolate exhibited no satelitic growth with Staph. aureus. It was non-motile, catalase-negative and oxidasepositive. Further tests were carried out in media containing 5% calfs serum, 5% ovine serum and 0.5% yeast extract. Urea, citrate, gelatin and M.R.-V.P. tests were negative. Indole was produced and nitrates reduced to nitrites. Glucose, mannose, maltose, xylose, trehalose, mannitol and sorbitol were fermented without gas production. Lactose, sucrose, dulcitol, arabinose, inositol, salicin, rhamnose, raffinose and galactose were not fermented. Cultural and biochemical characteristics indicate this organism to be identical to the Hemophilus-like organism isolated from cases of TEME. The micro-organism has been referred to as Hemophilus somnus (1).
Waldham et al (7) recently associated H. somnus with the birth of weak calves. These authors suggested that it may be a congenital infection in such calves since they frequently showed clinical signs at birth. We can only speculate with regard to the route of infection. We have isolated H. somnus
from purulent vaginal discharges of repeatbreeder cows. Direct invasion through the cervix is only one possible route of infection. Since the organism may cause a septicemia in adult animals, hematogenous infection of the placenta should also be considered. The significance of H. somnus in reproductive problems of cattle is not clear at this time. It is known that cows may carry the organism in their reproductive tracts. There is also a report of venereal transmission by an infected "marker" bull to virgin heifers (G. G. Klavano, personal communication). This observation could have serious implications in those herds where natural breeding or "marker" bulls are used. Serological surveys have also shown that the infection is widespread in areas where TEME occurs (3, 4). A more diligent search for H. somnus in aborted fetuses and placentas may raise the percentage of diagnoses due to infectious agents. Routine procedures in the examination of aborted fetuses, should include culturing of placentas and fetal stomach contents under 10% CO2 for at least 72 hours, particularly if Gram stains of the placenta revealed small Gram negative bacilli.
Summary H. somnus was isolated from the placenta, stomach contents and lung of seven and onehalf month old bovine fetus. The lesions in the Discussion fetus were characterized by vascular necrosis, The isolation of H. somnus from the placenta, thrombosis and leukocytosis in the brain, lung, fetal stomach contents and lung with septicemic myocardium and kidney. In addition, interstitial lesions in the placenta and several fetal organs pneumonia and myocarditis were prominent. strongly suggest that the abortion was due to The placental lesions consisted of thrombosis this organism. The vascular necrosis and vaseu- and medial necrosis of stromal arteries with litis with thrombosis in the placenta and fetal edema and placentitis. This appears to be the organs are similar to lesions associated with first recorded isolation of H. somnus from an H. somnus in older animals. aborted bovine fetus which showed septicemic All biochemical and sensitivity results of this lesions. isolate were similar to those of other H. somnus strains from a variety of bovine sources (G.A. Resume Garcia-Delgado, personal communication 1975). Les auteurs ont isole Hemophilus somnus du To our knowledge this is the first recorded iso- placenta, du contenu stomacal et des poumons lation of this organism from an aborted bovine d'un avorton bovin de sept mois et demi. Les fetus. Firehammer (2) reported the isolation of lesions de cet avorton se caracterisaient par de a Hemophilus organism from an aborted fetus la neerose vasculaire, de la thrombose et une and the dam's vaginal tract. The species was leucocytose du cerveau, des poumons, du myonot definitely identified and the biochemical carde et des reins; elles comprenaient aussi une characteristics reported did not correspond with pneumonie interstitielle et une myocardite. Le those of H. somnus. placenta presentait de la thrombose et de la 369
CANADIAN VETERINARY JOURNAL
n6crose de la media des arteres du stroma, ainsi que de roedeme et une placentite. II semble que ce soit la premiere fois qu'on rapporte l'isolation de H. somnus d'un avorton bovin presentant des lesions septicemiques. Addendum Since the preparation of this paper a case of bovine abortion associated with H. somnus was reported by D. W. Chladek. Am. J. vet. Res. 36: 1041. 1975. REFERENCES
1. BAILIE, W. E. Characterization of Hemophilus somnus (new species), a micro-organism isolated from infectious thrombo-embolic meningo-encephalomyelitis of cattle. Ph.D. Thesis. Kansas State University. 1969. 2. FnREAMMER, B. D. Bovine abortion due to Hemophilus species. J. Am. vet. med. Ass. 135:
421-422. 1959.
3. DmRKs, R. E., S. A. HANNA and R. C. DILL-
MAN. Epizootiology and pathogenesis of Hemophilus somnus infection. J. Am. vet. med. Ass. 163: 866-869. 1973. 4. KENNEDY, P. C., E. L. BIBERsEIN, J. A. HowARTH, L. M. FRAzIm and D. L. DUNGWORTH. Infectious meningo-encephalitis in cattle caused by a Hemophilus-like organism. Am. J. vet. Res. 21: 403-409. 1960. 5. SOLTYS, M. A. Bacteria and Fungi Pathogenic to Man and Animals. p. 498. London: Bailliere Tindall and Cox. 1963. 6. VAN DIREUMEL, A. A., R. A. CuRiTS and H. L. RUHNRE. Infectious thromboembolic meningo-encephalitis in Ontario feedlot cattle. Can. vet. J. 11: 125-130. 1970. 7. WALDHAM, D. G., R. F. HALL, W. A. MEINERSHAGEN, C. S. CARD and F. W. FRANK. Hemophilus somnus infection in the cow as a possible contributing factor to weak calf syndrome: Iso-
lation and animal inoculation studies. Am. J. vet. Res. 35: 1401-1403. 1974.
PUBLICIT9 CONCERNANT LES DROGUES D'APPLICATION
VETERINARY DRUG ADVERTISING
VgTgRINAIRE
The Canadian Veterinary Medical Association Drug Committee has advised the Bureau of Veterinary Medicine, Health Protection Branch of complaints received from veterinarians in connection with advertisements for veterinary drugs appearing in various Canadian publications or in literature distributed through the mails. Section 9 (1) of the Food and Drugs Act expressly prohibits the labelling, sale or advertising of "any drug in a manner that is false, misleading or deceptive or is likely to create an erroneous impression regarding its character, value, quantity, composition, merit or safety." Veterinarians who encounter advertisements or brochures for drugs intended for use in animals that they consider are a contravention of the Food and Drugs Act should contact: Bureau of Drug Surveillance Drugs Directorate Health Protection Branch Department of National Healh and Welfare Tunney's Pasture Ottawa, Ontario KlA 0L2 It will facilitate the work of the Branch if a copy of the advertisement, a reference to its source and a brief outline of the reasons for believing that the advertisement is deceptive are included in the communication.
Le Comite sur les medicaments de I'Association canadienne des Veterinaires a informe la Direction generale de la protection de la sante sur les drogues d'application veterinaire des plaintes formulees par les veterinaires au suiet d'annonces publicitaires parues dans diverses publications canadiennes ou dans des imprimes envoyes par courrier. L'article 9 (1) de la Loi des aliments et drogues interdit formellement 1'etiquetage, la vente ou la publicite de "quelque drogue de maniere fausse, trompeuse, ou mensongere, ou qui peut creer une fausse impression quant A la nature, valeur, quantite, composition, ou quant aux avantages ou a la surete de la drogue". Les veterinaires estimant qu'une reclame ou brochure concernant les drogues destinees aux animaux constitue une infraction a la Loi des aliments et drogues devraient s'adresser au: Bureau de la surveillance des m6dicaments Direction des drogues Direction g6n6rale de la protection de la santA MinistAre de la Sant6 et du Bien-;t.e social Parc Tunney Ottawa (Ontario) KIA 0L2 Pour faciliter le travail de la Direction, joindre a la lettre une copie de la r6clame, en indiquer la source et exposer brievement les raisons portant a croire qu'elle est mensongere.
370
