Author's Accepted Manuscript
A portable DNAzyme-based optical biosensor for highly sensitive and selective detection of lead (II) in water sample Nimet Yildirim, Feng Long, Miao He, Ce Gao, Han-Chang Shi, April Z. Gu
www.elsevier.com/locate/talanta
PII: DOI: Reference:
S0039-9140(14)00261-6 http://dx.doi.org/10.1016/j.talanta.2014.03.062 TAL14657
To appear in:
Talanta
Received date: 6 February 2014 Revised date: 25 March 2014 Accepted date: 26 March 2014 Cite this article as: Nimet Yildirim, Feng Long, Miao He, Ce Gao, Han-Chang Shi, April Z. Gu, A portable DNAzyme-based optical biosensor for highly sensitive and selective detection of lead (II) in water sample, Talanta, http://dx. doi.org/10.1016/j.talanta.2014.03.062 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
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A Portable DNAzyme-Based Optical Biosensor for Highly Sensitive and Selective Detection
2
of Lead (II) In Water Sample
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Nimet Yildirima,b, Feng Longb,c, Miao Hec, Ce Gaoa,b, Han-Chang Shic and April Z. Gua,b*
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a
Bioengineering Program, Northeastern University, Boston, USA bDepartment of Civil and
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Environmental Engineering, Northeastern University, Boston, USA cSchool of Environment and
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Natural Resources, Renmin Univerisity of China, Beijing, China, dDepartment of Environment
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Science and Engineering, Tsinghua University, Beijing, China
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Corresponding author: [email protected]
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Abstract: A portable, rapid and cost-effective DNAzyme based sensor for lead ions detection in
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water samples has been developed using an optical fiber sensor platform. The presence of Pb2+
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cleaves the DNAzymes and releases the fluorescent labeled fragments, which further hybridize
12
with the complementary strands immobilized on the optic fiber sensor surface. Subsequent
13
fluorescent signals of the hybridized fluorescent labeled fragment provides quantitative
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information on the concentrations of Pb2+ with a dynamic range from 2 to 75 nM with a
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detection limit of 1.03 nM (0.21 ng mL-1). The proposed sensor also shows good selectivity
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against other mono and divalent metal ions and thus holds great potential for the construction of
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general DNAzyme-based sensing platform for the monitoring of other heavy metal ions. The
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sensor can be regenerated with a 1 % SDS solution (pH 1.9) over 100 times without significant
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deterioration of the sensor performance. This portable sensor system can be potentially applied
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for on-site real-time inexpensive and easy-to-use monitoring of Pb2+ in environmental samples
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such as wastewater effluents or water bodies.
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Keywords: Pb2+, DNAzyme, biosensor, optical sensor, environmental analysis.
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1
Graphical Abstract
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1.
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Lead (Pb2+) is one of the most toxic metallic pollutants that can cause neurological, reproductive,
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cardiovascular, and developmental disorders even at very low levels (
A portable DNAzyme-based optical biosensor for highly sensitive and selective detection of lead (II) in water sample Nimet Yildirim, Feng Long, Miao He, Ce Gao, Han-Chang Shi, April Z. Gu
www.elsevier.com/locate/talanta
PII: DOI: Reference:
S0039-9140(14)00261-6 http://dx.doi.org/10.1016/j.talanta.2014.03.062 TAL14657
To appear in:
Talanta
Received date: 6 February 2014 Revised date: 25 March 2014 Accepted date: 26 March 2014 Cite this article as: Nimet Yildirim, Feng Long, Miao He, Ce Gao, Han-Chang Shi, April Z. Gu, A portable DNAzyme-based optical biosensor for highly sensitive and selective detection of lead (II) in water sample, Talanta, http://dx. doi.org/10.1016/j.talanta.2014.03.062 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
1
A Portable DNAzyme-Based Optical Biosensor for Highly Sensitive and Selective Detection
2
of Lead (II) In Water Sample
3
Nimet Yildirima,b, Feng Longb,c, Miao Hec, Ce Gaoa,b, Han-Chang Shic and April Z. Gua,b*
4
a
Bioengineering Program, Northeastern University, Boston, USA bDepartment of Civil and
5
Environmental Engineering, Northeastern University, Boston, USA cSchool of Environment and
6
Natural Resources, Renmin Univerisity of China, Beijing, China, dDepartment of Environment
7
Science and Engineering, Tsinghua University, Beijing, China
8
Corresponding author: [email protected]
9
Abstract: A portable, rapid and cost-effective DNAzyme based sensor for lead ions detection in
10
water samples has been developed using an optical fiber sensor platform. The presence of Pb2+
11
cleaves the DNAzymes and releases the fluorescent labeled fragments, which further hybridize
12
with the complementary strands immobilized on the optic fiber sensor surface. Subsequent
13
fluorescent signals of the hybridized fluorescent labeled fragment provides quantitative
14
information on the concentrations of Pb2+ with a dynamic range from 2 to 75 nM with a
15
detection limit of 1.03 nM (0.21 ng mL-1). The proposed sensor also shows good selectivity
16
against other mono and divalent metal ions and thus holds great potential for the construction of
17
general DNAzyme-based sensing platform for the monitoring of other heavy metal ions. The
18
sensor can be regenerated with a 1 % SDS solution (pH 1.9) over 100 times without significant
19
deterioration of the sensor performance. This portable sensor system can be potentially applied
20
for on-site real-time inexpensive and easy-to-use monitoring of Pb2+ in environmental samples
21
such as wastewater effluents or water bodies.
22
Keywords: Pb2+, DNAzyme, biosensor, optical sensor, environmental analysis.
1
1
Graphical Abstract
2 3
1.
4
Lead (Pb2+) is one of the most toxic metallic pollutants that can cause neurological, reproductive,
5
cardiovascular, and developmental disorders even at very low levels (
