Vox Sang. 28: 322-324 (1975)

A New Case of Recombination Within the HL-A System KONRADBERG,FRIEDRICH SCHWARZFISCHER AND HANSWISCHERATH Institut fur Anthropologie und Humangenetik der Universitat Munchen, Miinchen

Abstract. We report another case of recombination within the HL-A system between the first and second segregant series of antigens. The recombination frequency is 0.8%.

The human HL-A system is nowadays considered to consist of two loci, a third locus [l I] is currently discussed. Recombinations have been reported by different authors [I, 2, 4-10, 121. We describe a case of cross-over observed in a family from the ‘Bayrischer Wald’. HL-A typing was performed with the microlymphocytotoxicity test (NIH technique) using mono- and multispecific sera (Behring, Marburg). HL-A phenotype

HL-A genotype

Interpretation

Father Mother Child, Child, Child3 Child,

The individuals have been tested several times with identical results. Our serological testing showed no illegitimacy of the children (see table on the following page).

Received: August 12, 1974; accepted: September 2, 1974.

A,

A, 0

A,

0

Mother

Child,

Child,

Child,

Child,

0

Father

EcDEe P,-

MSS K-

K-

ccDEe P,+

MSs

MSS

KK-

K-

K-

K

ccDEe P,+ CcDee P,+

MSs

P,+

ccdee

MSS

P

CcDEe P,+

Rh

MSs

ABO MNSs

(a+b-)

(a+b-)

(a+b-)

(a+b-)

(a+b-)

(aSb-)

Fy

(a+b-)

(a+b+)

(a+b+)

(a+b+)

(a+b+)

(a+b-)

Ik

1-1

1-1

1-1

1-1

1-1

1-1

Hp

1-1

2-2

2-1

2-2

2-1

2-1

Gc

(1-2-)

(1-2-)

(1-2-)

(1-2-)

(1-2-)

(1-2-)

Gm

2-1 1-1 2-1 2-1 1-1 1-1

B B B B B

1-1

1-1

1-1

1-1

1-1

1-1

PGM AK

AE3

AcP

1-1

1-1

1-1

1-1

1-1

1-1

A

A

A

A

A

A

2-1

2-1

2-1

2-1

2-2

1-1

ADA PGD GPT

W N

w

5

2

0

324

BERGet al.

The biostatistical opinion of parentage based upon the results of blood group tests [3] demonstrates a probability of 99.9980-99.9975%, i. e. paternity practically proved. In the course of examination of 17 families with 63 children we found one recombination in 119 haplotypes, i. e. 0.8%. Our result corresponds to those of other authors. References 1 BODMER,W. F.; BODMER, J. G., and TRIPP,M. : Recombination between the LA and 4Loci of the HL-A system. Histocomp. Test., pp. 187-191 (Munksgaard, Copenhagen 1970). H. J.; TERASAKI, P.I., and GOOD,R. A. : Recombination 2 GATIT, R. A.; MEUWISSEN, within the HL-A locus. Tissue Antigens I: 239-241 (1971). 3 HUMMEL, K.: Biostatistische Abstammungsbegutachtung mit Blutgruppenbefunden (Fischer, Stuttgart 1973). F.; SVEJGAARD, A.; AHRONS, S., and STAUB-NIELSEN, L.: Crossing4 KISSMEYER-NIELSEN, over within the HL-A system. Nature, Lond. 224: 75-76 (1969). 5 KLOUDA,P. T. and LAWLER. S. D. : Another recombination within the HL-A system. Vox Sang. 22: 85-88 (1972). D.: Another case of recombination within the HL-A 6 MAYR,W. R. and MICKERTS, system. Tissue Antigens I: 47-48 (1971). 7 SEIciNALET, J.: A case of crossing over in the HL-A system. Transplantation I2: 224-225 (1971). 8 SOULIER, J. P. and PROU-WARTELLE, 0.: A new case of crossing over in the HL-A system. Tissue Antigens 2: 302-305 (1972). A.; BRATLIE, A.; HEDIN,P. J.; H ~ M A N C.;, JERSILD,C.; KBMEYER9 SVEJGAARD, B.; LINDHOLM, A.; Low, B.; MESSETER, L.; M ~ L L E R E.;, NIELSEN,F.; LINDBLOOM, SANDBERG, L. ; STAUB-NIELSEN, L., and THORSBY, E. : The recombination fraction of the HL-A system. Tissue Antigens I: 81-88 (1971). A.; STAUB-NIELSEN, L. ; RYDER, L. P. ; KISSMEYER-NIELSEN, F. ;SANDBERG, 10 SVEJGAARD, E.: Subdivisions of HL-A antigens. Evidence of a L.; LINDHOLM, A., and THORSBY, ‘new’ segregant series. Histocomp. Test., pp. 465-473 (Munksgaard, Copenhagen 1972). 11 WALFORD,R. L.; WATERS,H., and SMTH, G. S. : Human transplantation antigens. Fed. Proc.29: 2011-2017 (1970). 12 WARD, F. E.; SOUTHWORTH,J. G., and AMOS, D. B.: Recombination and other chromosomal aberrations within the HL-A locus. Transplant. Proc. I: 352-356 (1969).

Dr. Dr. H. WISCHERATH,Institut fur Anthropologie und Humangenetik der Universitit Miinchen, Richard-Wagner-Strasse 10 I, 0-8000 MCtzchen (BRD)