Anal Bioanal Chem DOI 10.1007/s00216-014-7651-9
RESEARCH PAPER
A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system Li-Li Huang & Yong-Jie Jin & Dongxu Zhao & Chao Yu & Jian Hao & Hai-Yan Xie
Received: 18 December 2013 / Revised: 21 January 2014 / Accepted: 21 January 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83±4.3% (mean±SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification. Keywords Sialic acid . Phenyl-boronic acid . Vesicular stomatitis virus . Recognition . Labeling
Introduction Fluorescence labeling of viruses is of great importance for tracking living viruses and detecting viral pathogens. There are some necessary requirements for virus labeling, such as no Electronic supplementary material The online version of this article (doi:10.1007/s00216-014-7651-9) contains supplementary material, which is available to authorized users. L.
RESEARCH PAPER
A fast and biocompatible living virus labeling method based on sialic acid-phenylboronic acid recognition system Li-Li Huang & Yong-Jie Jin & Dongxu Zhao & Chao Yu & Jian Hao & Hai-Yan Xie
Received: 18 December 2013 / Revised: 21 January 2014 / Accepted: 21 January 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract The sialic acid (SA)-phenylboronic acid (PBA) recognition system is of particular interest in the bioconjugation field, because it is simple, fast, efficient, and biocompatible. In this paper, we report a novel method for reversibly labeling living virus with quantum dots (QDs) by taking advantage of this SA-PBA recognition system. The QDs were initially modified with PBA (QDs-PBA) to target them to the surface of vesicular stomatitis virus (VSV), which has abundant with SA on its envelope. The QDs-PBA was of good monodispersity and strong fluorescence, and could be conjugated with VSV by simply incubating with native VSV for 10 min at 37 °C, producing QDs-VSV that was capable of being imaged at the single virion level. The labeling efficiency attained 83±4.3% (mean±SD); meanwhile, the activity and recognition ability of the labeled virus were minimally affected. This method was simple, rapid, and reversible. This work promotes the virus labeling development to a new step. That is, native viruses can be reversibly labeled without any modification. Keywords Sialic acid . Phenyl-boronic acid . Vesicular stomatitis virus . Recognition . Labeling
Introduction Fluorescence labeling of viruses is of great importance for tracking living viruses and detecting viral pathogens. There are some necessary requirements for virus labeling, such as no Electronic supplementary material The online version of this article (doi:10.1007/s00216-014-7651-9) contains supplementary material, which is available to authorized users. L.
