ANALYTICAL

BIOCHEMISTRY

r),

470-501

(1977)

A Compilation of Amino Acid Analyses of Proteins Xl. Residues

per Thousand

Residues,

3

DONALD M. KIRSCHENBAUM" department of Bioc~emisf~, Downstate Medical Center-SUNY, Brooklyn, New York 11203 Received October 29, 1976; accepted January 6, 1977

This paper, the third in a series (l), is a compilation of amino acid analyses of 312 proteins or proteinaceous materials which are listed in Table 1 by name and with their respective sources. In Table 2, the compositions data are reported as residues of amino acid per 1000 residues. The data were given in the references cited in various ways, e.g., percentage of moles of amino acid recovered, moles per 100 moles of amino acids, and percentage of residues. All such values have been changed to residues per 1000 residues, so that comparisons of the compositional data may be made. In addition to the amino acid analyses, the footnotes contain analytical values for carbohydrate, e.g., glucose, glucosamine, galactose, galactosamine, and sialic acid, and for amino acids not listed in Table 2 e.g., hydroxylysine and hydroxyproline. All analytical data reported were determined by column chromatography except for some tyrosine, tryptophan, and occasional half-cystine values. ACKNOWLEDGMENTS The Library of The Downstate Medical Center College of Medicine has been the major source of all publications examined. What was not avaiiable in the library was obtained for me from other libraries. I should like to thank the librarians for their most useful and #ntinuing assistance. During the summer of 1971- 1975, I was a Library Reader at the Library of the Marine Biological Laboratory, Woods Hole, Mass. I should like to thank the librarians of that library for their assistance. I thank Mr. E. Becker and Mr. D. Siegel for editing assistance. I thank Miss M. Colindreler, Mrs. F. Kuller, and Mrs. E. Levine for typing assistance.

REFERENCE 1. Kirschenbaum,

FOR TEXT

D. (1973) Anal. Biochem. 53,223; 56,208.

REFERENCES

FOR TABLE 2

1. Johnson, A. W., Wilhelm, .I. A., Ward, D. N., Hnilica, L. S. (1973) Biochim. Acta 295, 140. 2. Kobayashi, K. (1971) Immunochemistry 8,785. * Faculty Exchange Scholar, State University

Biophys.

of New York.

470 Copyright 0 1977 by Academic Press. Inc. AR rights of reproduction in any form reserved.

ISSN WQ3-2697

AMINO

ACID ANALYSES

OF PROTEINS

XI

471

3. Schrohenloher, R. E., Mestecky, J., and Stanton, T.-H. (1973) Biochim. Biophys. Actu 295, 576. 4. McIntosh, R. M., Kulvinskas, C., and Kaufman, D. B. (1971)Inr. Arch. Allergy 41,700. 5. Saha, A., Sambury, S., Smart, K., Heiner, D. C.. Sargent, A., and Rose, B. (1%9) J. Immunol. 102,476. -6. Schwick, H. G., Heimburger, N., and Haupt, H. (1966) 2. Inr. Med. 21, 193. 7. Heimburger, N., Haupt, H., and Schwick, H. G. (1971) in Proceedings of the International Research Conference on Proteinase Inhibitors (Fritz, H., and Tschesche, H., eds.), p. 1, W. deGruyter, Berlin. 8. Hamberg, U., Stelwagen, P., and Et-vast, H. S. (1973) Eur. J. Biochem. 40,439. 9. Hochstrasser, K., Reichert, R., Schwan, S., and Werle, E. (1974) Hoppe-Seyler’s 2. Physiol. Chem. 354,923. 10. Hochstrasser, K., Feuth, H., and Steiner, 0. (1974) Hoppe-Seyler’s Z. Physiol. Chem. 354, 927. 11. Crewther, W. G., Dobb, M. G., Dowling, L. M., and Hat-rap, B. S. (1968) in Symposium on Fibrous Proteins (Crewther, W. G., ed.), p. 329, Butterworths, Reading, Mass. 12. Cotfield, M. C., Fletcher, J. C., and Robson, A. (1968) in Symposium on Fibrous Proteins (Crewther, W. G., ed.), p. 299, Butterworths, Reading, Mass. 13. Hermier, J., Lebeault, J. M., and Zevaco, C. (1970) Bull. Sot. Chim. Biol. 52, 1089. 14. Nagaoka, K., and Yamada, Y. (1974) Agr. Biol. Chem. 37, 2791. 15. Simons, K., Ehnholm, C., Renkonen, O., and Bloth, B. (1970) Acta Path. Microbial. Stand. 78B, 459. 16. Fidge, N. (1973) Biochim. Biophys. Acta 295, 258. 17. Shore, V. G., and Shore, B. (1973) Biochemistry 12, 502. 18. Havel, R. J., and Kane, J. P. (1973) Proc. Nat. Acad. Sci. USA 70, 2015. 19. Shore, V. G., Shore, B., and Hart, R. G. (1973) Biochemistry 13, 1579. 20. Ayrault-Jarrier, M., Lastra, G., Pastier, D., Burdin, J., and Polonovski, J. (1972) Biochimie 54, 973. 21. Blaton. V., Peeters, H., Vercaemst, R., Vandecasteele, N., and Caster, H. (1974) Biochemistry 13, 1127. 22. Kostner, G. (1972) Biochem. J. 130, 913. 23. Swann, D. A., and Radin, E. L. (1972) J. Biol. Chem. 247, 8069. 24. Stimson, W. H., and Scott, L. E. (1972) FEBS Letf. 23, 298. 25. Cassman, M., and Vetterlein, D. (1974) Biochemistry 13, 684. 26. Siegel, L., and Englard, S. (1962) Biochim. Biophys. Acru 64, 101. 27. Marshall, C. L., Wicken, A. J., and Brown, A. D. (1969) Canad. J. Biochem. 47, 71. 28. Stoeckenius, W., and Kunau, W. H. (1%8) J. Cell Biol. 38, 337. 29. (a) Bakerman, S., and Wasemiller, G. (1%7)Biochemistry 6, 1100; (b) Tanner, M. J. A., and Gray, W. R. (1971) Biochem. J. 125, 1109; (c) Chapman, D. (1972) in Polymerization in Biological Systems, Ciba Foundation Symposium No. 7, p. 261, Elsevier, Amsterdam; (d) Palmer, F. B. St. C., and Verpoorte, J. A. (1971) Canad. J. Biochem. 49, 337. 30. Hamaguchi, H., and Cleve, H. (1971) Biochim. Biophys. Acra 233, 320. 31. Passow, H. (1969) Progr. Biophys. Mol. Biol. 19, 425. 32. (a) Zwaal,R. F. A., andVanDeenen, L. L. M. (1968)Biochim. Biophys. Acra 150,323; (b) Rosenberg, S. A., and Guidotti, G. (1%9) J. Biol. Chem. 244, 5118. 33. Mazia, D., and Ruby, A. (1968) Proc. Nat. Acad. Sci. USA 61, 1005. 34. (a) Pellett, P. L., Pascoe. E., and Shaafi, R. I. (1972) Comp. Biochem. Physiol. 41B, 483; (b) Kobylka, D., and Carrway, K. L. (1972b) Biochim. Biophys. Actu 288, 282. 35. Mattel, M. B., Dubois, P., and Got, R. (1973) Biochim. Biophys. Acru 311, 565. 36. Chien, H. C., and Richardson, T. (1967) J. Dairy Sci. 50, 1868. 37. Brunner, J. R. (1967) in Structural and Functional Aspects of Lipoproteins in Living Systems (Tria, E., and Scanu, A. M., eds.), p. 545, Academic Press, New York.

472

DONALD

M. KIRSCHENBAUM

38. Spenny, J. G., Saccomani, G., Spitzer, H. L., Tomana, M., and Sachs, G. (1974)Arch. Biochem. Biophys. 161,456. 39. Mahieu, P. M., and Winand, R. J. (1973) Eur. J. Biochem. 37, 157. 40. Mahieu, P. M., and Winand, R. J. (1970) Eur. J. Biochem. 12,410. 41. Marquardt, H., Wilson, C. B., and Dixon, F. J. (1973) Biochemistry 12, 3260, 42. Westberg, N. G., and Michael, A. F. (1970) Biochemistry 9, 3837. 43. Beisswenger, P. J., and Spiro, R:G. (1970) Science 168, 5%. 44. (a) Kefalides, N. A. (1970a) in Chemistry and Molecular Biology of Interce~ular Matrix (Balazs, E. A., ed.), Vol. 1, p. 535, Academic Press, New York; (b) Funs, K. K., and Kalant, N. (1972) Biochem. J. 129, 733. 45. Kefalides, N. A. (1%8) Biochemistry 7, 3103. 46. Hudson, B. G., and Spiro, R. G. (1972) J. Biol. Chem. 247,4229. 47. Bomens, M., and Kasper, C. B. (1973) J. Bid. Chem. 248, 571. 48. Barber, A. J., and Jamieson, G. A. (1970) J. Biol. Chem. U&6356. 49. Meldolesi, J., and Cova, D. (1972) J. Cell Eiol. SJ, 1. 50. Hjerten, S., and Johansson, K. E. (1972) Biochim. B~ophys. Acta 288,3 12. 5 1. Weser, U., Rupp, H., Donay, F., Linnemann, F.. Voelter, W., Voetsch, W., and Jung, G. (1973) Eur. J. Biochem. 39, 127. 52. Winge, D. R., and Rajagopalan, K. V. (1972)Arch. Biochem. Biophys. 153, 755. 53. Kagi, J. H. R. (1970) Eighth-International Congress of Biochemistry, Switzerland Abstracts, p. 130. 54. Surguchev, A. P., Surgucheva, I. G., Ermorhina, T. M., and Zaitseva, G. N. (1971) *Eiokhimiyh 36, 791. 55. Tokuyama, H. ‘(1973) Blochim. Biophys. Actu 317, 338. 56. Hunt, S., and Jevons, F. R. (1965) B~ochem. J. 97,701. 57. DeSalegui, M., and Plonska, H. (1%9) Arch. Biochem. Biophys. 129, 49. 58. Rossi, E., and Stall, E. (1968) in Biochemistry of Glycoproteins and Related Substances, Part 2, pp. 72-73, S. Karger, Basel. 59. Lombart, C., and Winzler, R. J. (1972) Biochem. J. 128, 975. 60. Huser, H., Mody, E., and Faillard, H. (1973)Hoppe-Seyler’sZ. Physiol. Chem. 354,749. 61. Neiderhiser, D. H., Plantner, J. J., and Carlson, D. M. (1971)Arch. Biochem. Biophys. 145, 155. 62. Degand, P., Roussel, P., Lamblin, G., and Havez, R. (1973) Biochim. Biophys. Acta 320, 318. 63. Lamblin, G., Lhermitte, M., Degand, P., Sergeant, Y. H., and Roussel, P. (1973) Biochim. Biophys. Acta 322,372. 64. Tung, K. K., Rosenthal, A., and Nordin, J. H. (1971) J. Biol. Chem. 246,6722. 65. Wolfram, F., and Kotorii, K. (1968) J. Neurochem. l&1281; see also Eng, L. F., Chao, F. C., Gerstl, B., Pratt, D., and Tavastsjema, M. G. (1968) Biochemistry 7, 4455. 66. Wolfram, F., and Tourtellotte, W. W. (1972) Neurology 22, 1044. 67. (a) Hegstrand, L. R., and Komguth, S. E. (1973) Biochim. Biophys. Acta 317, 380; (b) Deibler, G. E., Martenson, R. E., and Kies, M. W. (1972) Prep. Biochem. 2, 139. 68. Puett, D., Friebele, E., and Hammonds, Jr., R. G. (1973) Biochim. Biophys. Acru 328, 261. 69. Hayashi, Y. (1972) J. Biochem. (Tokyo) 72,83. 70. Mani, R. S., and Kay, C. M. (1973) Canad. J. Biochem. 51, 178. 71. Bodwell, C. E., Hepner, P. A., Brooks, B., and Hagan, S. (1971)Int. J. Biochem. 2,682. 72. LeStourgeon, W. M., and Rusch, H. P. (1973) Arch. Biochem. Biophys. 155, 144. 73. (a) Olson, M. 0. J., Orrick, L. R., Jones, C., and Busch, H. (1974) J. Biol. Chem. 249, 2823; (b) Grogan, D. E., Desjardins, R., and Busch, H. (1%6) Cancer Res. 26,775; (c) Teng, C. S., Teng, C. T., and Allfrey, J. G. (1971) J. Biol. Chem. 2.46, 3597. 74. Neu, H. C. (1%7) J. Biol. Chem. 242,38%.

AMINO

ACID ANALYSES

OF PROTEINS

XI

473

75. Degens, C. T., Spencer, D. W., and Parker, R. H. (1967) Comp. Biochem. Physiol. 20, 553. 76. Meenakshi, V. R., Hare, P., and Wilbur, K. M. (1971) Comp. Biochem. Physiol. 40B, 1037. 77. Degens, E. T., and Love, S. (1965) Nature (London) 205,876. 78, Carmichael, D. .I., Dodd, C. M., and Nawrot, C. F. (1974) C&if. Tissue Res. 14, 177. 79. Sleigh, R. W., Melrose, G. J. H., and Smith, M. B. (1973)~~~~h~rn. Biophys. Acfu 310, 453. 80. Skinner, M., and Cohen, A. S. (1973) Biochem. Biophys. Res. Commun. 54, 732. 81. Anstee, D. J., and Pardoe, G. I. (1973) Eur. J. Biochem. 39, 149. 82. Halsey, J. F., and Harrington, W. F. (1973)Biochemistry 12, 693. 83. Havir, E. A., and Hanson, K. R. (1973) Biochemistry 12, 1583. 84. Beuving, G., and Gmber, M. (1971) Biochim. Biophys. Acta 232, 524. 85. Yamamoto, S., Minoda, Y., and Yamada, K. (1972) Agr. Biol. Chem. 36, 2097. 86. Yurewicz, E. C., Ghalambor, M. A., Duckworth, D. H., and Heath. E. C. (1971)5. B&Z. Chem. 246,X07. 87. Baden, H. P., Goldsmi~, L. A., and Fleming, B. (1973)Biockim. ~~~phys. Actu 317,303. 88. Berg, R. A., and Prockop, D. J. (1973) Biochemistry 12, 3395. 89. Thompson, H. C., and Thompson, M. H. (1970) Comp. Biochem. Physiol. 35,471. 90. Yui. C. (1971) J. Biochem. (Tokyo) 69, 101. 91. Ohkawa, I., Kageyama, M., and Egami, F. (1973) J. Biochem. (Tokyo) 73, 281. 92. Tono, H., and Komberg, A. (1967) .I. Biol. Chrm. 242, 2375.

474

DONALD

M. KIRSCHENBAUM TABLE PROTEIN

Number 1 Name Source

2 Name Source 3

Name Source

4 Name Source 5

6

Name .Source Name Source

7 Name Source

8 Name Source

9 Name Source 10

11

Name Source Name

12

Source Name

13

Source Name

14

Source Name

15

Source Name Source

16 Name Source

1 INDEX

Number Histone, fraction 1 Sea urchin (Strongylo-

17 Name

centrotus pluteus)

18

putpuratus

nuclei Histone, fraction 2 Sea urchin (S. purpuratus pluteus) nuclei Histone, fraction 3 Sea urchin (S. purpuratus pluteus) nuclei Histone, fraction 4 Sea urchin (S. purpuratus pluteus) nuclei Histone, fraction 5 Sea urchin (S. purpuratus pluteus) nuclei Histone, fraction 6 Sea urchin (S. purpuratus pluteus) nuclei Immunoglobulin, secretory piece, bound Human colostrum Immunoglobulin, J chain Human myeloma IgA protein Fe1 Immunoglobulin, IgG Human supemate serum after cryoprecipitation Immunoglobulin, IgG Human, pooled Inhibitor, proteinase: a,-anti-trypsin Human serum Inhibitor, proteinase: o-anti-chymotrypsin Human serum Inhibitor, proteinase: IIntero-trypsin inhibitor Human serum Inhibitor, proteinase: anti-thrombin Human serum Inhibitor, proteinase: Cl-inactivator Human sentm Inhibitor, proteinase: cy,-macroglobulin Human serum

19

Source Name Source Name Source

20 Name Source

21 Name Source

22 Name Source

23 Name Source

24 Name Source

25 Name Source

26 Name Source

27 Name Source

28 Name Source

29 Name Source

30 Name Source

31 Name Source

32 Name Source

Source

34 Name Source

Inhibitor, proteinase Human bronchial mucus Inhibitor, proteinase Human plasma Kerateine, S-carboxymethyl-, (SCMK-A) a-Keratin of Merino wool y-Keratose Oxidized wool Leucine dehydrogenase Bacillus

subtilis

Lipase, F-3A Mucor

lipolyticus

Lipase, F-3B Mucor

lipolyticus

Lipoprotein, Lp (a) Human plasma Lipoprotein, very low density Pig plasma Lipoprotein, very low density, Band B Human plasma Lipoprotein, very low density, Band C Human plasma Lipoprotein, very low density, Band D Human plasma Lipoprotein, very low density, Band E Human plasma Lipoprotein, very low density, Band F Human plasma Lipoprotein, very low density, Band H Human plasma Lipoprotein, very low density, arginine-rich Human (with dysbetalipoproteinemia) Lipoprotein, very low density, arginine-rich Human (hyperlipemic) Lipoprotein, very low density Rabbit serum

AMINO

ACID ANALYSES TABLE

Source

36 Name 31

Source Name Source

38 Name Source

39 Name 40 41 42

Source Name Source Name Source Name Source

43 Name

44

Source Name Source

45 Name Source

46 Name Source

47 Name Source

48 Name 49

50

51 52 53

Source Name Source Name Source Name Source Name Source Name Source

475

XI

1 (Continued) Number

Number 35 Name

OF PROTEINS

Lipoprotein, very low density Rabbit serum, cholesteremic Lipoprotein, low density U-J%) Rabbit serum Lipoprotein, low density U-DU Rabbit serum, cholesteremic Lipoprotein, low density U-DL,) Rabbit serum, cholesteremic Lipoprotein, low density (U) Pig plasma Lipoprotein, low density(D) Pig plasma Lipoprotein, low density Human plasma Lipoprotein 2, high density W) Pig plasma Lipoprotein 2, high density UN Pig plasma Lipoprotein 3, high density WI Pig plasma Lipoprotein, apo-high density, CYP Human serum Lipoprotein, soluble, aP, Lipoprotein #45 Lipoprotein, gellable, CUP, Lipoprotein #45 Lipoprotein, apo-high density (HDL,) Chimpanzee plasma Lipoprotein, apo-high density (HDL,) Chimpanzee plasma Lipoprotein B Human serum HDL, Lubrication factor Bovine synovial fluid a-Macroglobulin Human (female) serum Malic dehydrogenase, form a Bovine heart, cytoplasmic

54 Name Source

55 Name Source

56 Name Source

57 Name Source

58 Name Source

59 Name Source

60a Name 60b

Source Name Source

6Oc Name Source 61 Name Source

62 Name Source

63 Name 64

65 65a 66

Source Name Source’ Name Source Name Source Name Source

66a Name Source

67 Name Source

88a Name Source 68b Name Source

69 Name Source

Malic dehydrogenase, form b Bovine heart, cytoplasmic Malic dehydrogenase Bovine heart, mitochondria Malic dehydrogenase Bovine heart, supemate Membrane protein, defatted Halobacterium

halobium

cell envelope Membrane, purple H. halobium

Membrane, gas vacuole, (inttacytoplasmic) H. halobium

Membrane protein Human erythrocyte Membrane Human erythrocyte Membrane Human erythrocyte Membrane, water soluble protein Human erythrocyte Membrane protein Human erythrocyte Membrane protein Human erythrocyte Membrane Human erythrocyte Membrane Human erythrocytes Membrane Bovine erythrocytes Membrane Cat (Felis domesticus) erythrocyte Membranes, fat globules Bovine milk Membrane, fat globules Human milk Membranes, peak I Canine gastric cell Membranes, peak II Canine gastric cell Membranes, glomexular basement Human kidneys

476

DONALD

M. KIRSCHENBAUM

TABLE Number Membranes, glomerular basement Source Human kidneys 71 Name Membranes, tubular basement Source Human kidneys 72 Name Membranes, glometular basement Source Human kidneys 73 Name Membranes, glometular basement Source Human kidneys 74 Name Membranes, glomerular basement Source Human kidneys, diabetes mellitus 75 Name Membranes, glomerular basement Source Human kidneys 76 Name Memb~nes, gIome~lar basement Source Canine kidneys 77 Name Membranes, glomerular basement Source Ovine kidneys 78 Name Membranes, glometular basement Source Rat kidneys 78a Name Membranes, glometular basement Source Rat kidney 78b Name Membranes, glomerular basement Source Rat kidney, antiserum nephrosis 79 Name Membranes, glomerular basement Source Bovine kidneys 80 Name Membranes, nuclear Source Rat liver 81 Name Membranes Source Human platelets 82a Name Membranes, zymogen granule Source Guinea pig pancreas 82b Name Membranes, rough microsomes Source Guinea pig pancreas 82~ Name Membranes, smooth mierosomes

1 (Co~finued) Number

70 Name

83 84 85 86 87 88

88a 89

Source Name Source Name Source Name Source Name Source Name Source Name Source Name Source Name Source

Guinea pig pancreas Membranes Acholeplasma

Me~~o~ionein Rat liver Metallothionein Rat liver Met~lo~ionein Chicken liver Metallothionein Horse liver Methionyl-RNA BuciEius

laidlawii

synthetase

brevis

Microsomes Rabbit muscle Mitogen, fraction E-2 Shoriku (Phytolacca esculenta)

90 91

Name Source Name Source

92 93 94 95 96

Name Source Name Source Name Source Name Source Name Source

97 98 99

Name Source Name Source Name Source

100

101 102

Name Source Name Source Name Source

103 Name 104

Source Name Source

Mitogen, fmction E-3 Shoriku (P. esculenta) Mucin Whelk (Buccinum undatum) hypobranchial Mucin, major Porcine submaxillary gland Mucin, minor Porcine submaxillary gland Mucin Porcine submaxillary gland Mucin Canine submaxillary gland Mucin Canine submaxillary gland Mucin, major Bovine submaxillary gland Mucin, minor Bovine submaxillary gland Mucin, major Ovine submaxillary gland Mucin, minor Ovine submaxillary gland Mucin, Gottschalk Ovine submaxillary gland Mucin Bovine sublingual gland Mucin Equine submaxillary gland Mucin A Porcine gall bladder

AMINO

ACID ANALYSES TABLE

Number 105

106 107 108 109

Name Source Name Source Name Source Name Source Name Source

110 Name 111

Source Name Source

112 Name 113 114 115 116 117 118

Source Name Source Name Source Name Source Name Source Name Source Name Source

119 Name 120

Source Name Source

121 Name Source

122 Name Source

123 Name Source

124 Name Source

124a Name 125

Source Name Source

OF PROTEINS

477

XI

1 (Continued) Number

Mucin H Porcine gall bladder Mucin (Fucomucine) Human bronchiogenic cyst Mucin (Sulfomucine) Human bronchiogenic cyst Mucin (Sialomucine) Human bronchiogenic cyst Mucin, neutral (from Sepharose) Human bronchial Mucin, neutral (from CMcellulose) Human bronchial Mucin, neutral (from CMcellulose) Human bronchial Mycodextranase, fast Penicillium

127 128 129 130 131 132 133

Source Name Source Name Source Name Source Name Source Name Source Name Source Name Source

134 Name Source

135 Name

melinii

Mycodextranase,

126 Name

slow

P. melinii

Myelin Human centrum ovale Myelin Monkey centrum ovale Myelin Bovine centrum ovale Myelin Bovine optic nerve Myelin Bovine spinal cord white matter Myelin Human brain white matter Myelin Human brain MS white matter Myelin Human brain plaque edges Myelin Human brain plaque Myelin, basic protein Bovine, adult Myelin, basic protein Bovine, fetal Myelin, basic protein Bovine spinal cord Myoglobin I Turtle heart

Source

136 Name Source

137 Name Source

138 Name Source

139 Name Source

140 Name Source

141 Name Source

142a Name Source

142b Name Source

142~ Name Source

143 Name Source

144 Name

Myoblobin II Turtle heart Myoglobin III Turtle heart Myoglobin I Bovine heart Myoglobin II Bovine heart Myoglobin III Bovine heart Myoglobin IV Bovine heart Myosin, g-subunit Rabbit skeletal muscle Myosin, light chain (LC,) Rabbit cardiac muscle Myosin, light chain (LC,) Rabbit cardiac muscle Myosin Bovine muscle Myosin Porcine muscle Myosin Rabbit muscle Myosin Human uterus smooth muscle Myosin Human pectoral striated muscle Nuclei, phenol-soluble fraction Physarum

polycephalum

plasmodia Nucleoli, phenol-soluble fraction P. polycephalum plasmodia Nucleoli Rat Novikoff hepatoma ascites Nuclei, phenol-soluble protein fraction Rat liver Nuclei, phenol-soluble protein fraction Rat kidney 5’-Nucleotidase Escherichia

coli

Organic matrix

478

DONALD

M. KIRSCHENBAUM

TABLE Number Source

145 Name Source

146 Name Source

147 Name Source

148 Name Source

149 Name Source

150 Name Source

151 Name Source

152 Name Source

153 Name Source

154 Name Source

155 Name Source

156 Name Source

157 Name 158 159 168 161 162 163

Source Name Source Name Source Name Source Name Source Name Source Name Source

164 Name 165 166 167 168

Source Name Source Name Source Name Source Name

1 (Conrinued) Number

Argonauta

hians

egg

case

Organic matrix Loligo pen Organic matrix Nautilus

Source

170 Name

pompilius

Organic matrix Sepia cuttle bone Organic matrix Spirula spirula shell Organic shell matrix Achatinella

lorata

Organic shell matrix Acmaea

pustulata soluta willcoxi

Architectonica

nobilis

Organic shell matrix caelata

Organic shell matrix Bulla

Source

173 Name Source

174 Name 175 Name 176 Name Source

Organic shell matrix

Astraea

Source

172 Name

Source

Organic shell matrix Aplysia

Source

171 Name

Source

Organic shell matrix Akera

Janthina

169 Name

177 Name Source

178 Name Source

179 Name Source

striata

Organic shell matrix Cavolina

tridentata

Organic shell matrix Colus

trophius

Organic shell matrix Crepidula

fornicata

Organic shell matrix Crepidula

plana

Organic shell matrix Cypraea

scapula

Organic sheU matrix Epitonium

ongulatum

Organic shell matrix Fissurella

barbadensis

(from Puerto Rico) Organic shell matrix F. barbadensis (from Cuba) Organic shell matrix Gastropod (indefinable) Organic shell matrix Haliotis

crachfordi

Organic shell matrix Helisoma

Source

181 Name Source

182 Name Source

183 Name Source

zebra

Organic shell matrix Dolabella

180 Name

trivalvis

Organic shell matrix

184 Name Source

185 Name Source

186 Name Source

187 Name Source

188 Name Source

189 Name Source

190 Name Source

191 Name

janthina

Organic shell matrix Littorina

littorea

Organic shell matrix Lunatia

trisseriata

Organic shell matrix L. trisseriata operculum Organic shell matrix Melanella

martini

Organic shell matrix Murex

brevtfrons

Organic shell matrix Nerita

plexa

Organic shell matrix N. plexa operculum Organic shell matrix Nassarius

trivittatus

Organic shell matrix Oxynoe

viridis

Organic shell matrix Planorbis sp. Organic shell matrix Polinices

duplicatus

(Provincetown, Mass.) Organic shell matrix P. duplicatus (Texas) Organic shell matrix P. duplicatus (Texas) Organic shell matrix P. duplicatus (Florida) Organic shell matrix P. duplicatus (North Carolina) Organic shell matrix Siphonaria

alternata

Organic shell matrix Succinea

ovalis

Organic shell matrix Turitella

terebra

Organic shell matrix Umbraculum

indicum

(Indonesia) Organic shell matrix CJ. indicum (Hawaii) Organic shell matrix Urosalpinx

cinera

Organic shell matrix Viviparus

georgianus

Organic shell matrix

AMINO

ACID ANALYSES TABLE

Number Source

192 Name Source

193 Name Source

194 Name Source

195 Name 1%

Source Name Source

197 Name Source

198 Name 199 200 201

Source Name Source Name Source Name Source

202 Name Source

203 Name 204 205

Source Name Source Name Source

206 Name Source

207 Name 208 209

Source Name Source Name Source

210 Name

OF PROTEINS

479

XI

1 (C~~~j~~e~) Number

Aequipecten

irradians

(Woods Hole, Mass.) Organic shell matrix A. irradians (Woods Hole, Mass.) Organic shell matrix Anadara

transversa

(Woods Hole, Mass.) Organic shell matrix A. transversa (Mississippi Delta) Organic shell matrix A. transversa (Texas) Organic shell matrix A. transversa (Mississippi Delta) Organic shell matrix A. transversa (Campeche, Mexico) Organic shell matrix Arctica

Organic shell matrix consobrina

Organic shell matrix Crassostrea

virginica

Organic shell matrix Laevicardium

hyalina

Organic shell matrix Macoma tenta (Woods Hole, Mass.) Organic shell matrix M. tenta (Woods Hole, Mass.) Organic shell matrix Malletia sp. Organic shell matrix Mercenaria

212 Name Source

213 Name Source

214 Name Source

215 Name Source

216 Name Source

217 Name Source Source

219 Name Source

220 Name Source

221 Name Source

222 Name Source

compressus

Organic shell matrix Lyonsia

Source

mortoni

(Woods Hole, Mass.) Organic shell matrix L. mortoni (Woods Hole, Mass.) Organic shell matrix Limopsis

211 Name

218 Name

islandica

Corbicula

Source

mercenaria

Organic shell matrix Mulina laieralis (Salem, Mass.) Organic shell matrix

223 Name Source

224 Name Source

225 Name Source

226 Name Source

227 Name Source

228 Name Source

lateralis (Woods Hole, Mass.) Organic shell matrix M. lateralis (Woods Hole, Mass.) Organic shell matrix M. lateralis (Woods Hole, Mass.) Organic shell matrix M. lateralis (New York) Organic shell matrix M. lateralis (Long Island, N.Y.) Organic shell matrix M. lateralis (SapelloIsland, Ga.) Organic shell matrix M. lateralis (Louisiana) Organic shell matrix M. lateralis (Louisiana) Organic shell matrix M. lateralis (Texas) Organic shell matrix M. lateralis (Texas) Organic shell matrix M. lateralis (Compeche, Mex.) Organic shell matrix Mytilus edulis (Woods Hole, Mass.) Organic shell matrix M. edulis (Woods Hole, Mass.) Organic shell matrix M.

Neotrigonia

margaritacea

Organic shell platrix Nucula proxima (Woods Hole, Mass.) Organic shell matrix N. proxima (Woods Hole, Mass.) Organic shell matrix Nucula truncula (Woods Hole, Mass.) Organic shell matrix N. proxima (Woods Hole, Mass.) Organic shell matrix Per~ploma

leanum

DONALD

480

M. KIRSCHENBAUM

TABLE

Number

Number 229 230 231

Name Source Name Source Name Source

232

Name Source

233

Name Source Name Source Name Source

234 235

236

237

Name Source Name Source

238a Name 238b

pholad~ormis

Organic shell matrix Pitar cordata (Texas) Organic shell matrix Pitar morrhuana (Woods Hole, Mass.) Organic shell matrix P. morrhuana (Woods Hole, Mass.) Organic shell matrix Sax~domus

Source

L. irrorata

239b Name

Source

242b Name Source

243a Name Source

243b Name Source

244 Name

nuttali

Organic shell matrix Tagelus divisus (Bermuda) Organic shell matrix T. divisus (Long Island, N.Y.) Organic shell matrix Yoldia limatula (Woods Hole, Mass.) Organic shell matrix Y. limatula (Woods Hole, Mass.) Organic matrix protein, insoluble Littorina

Source

24Qa

Organic shell matrix Petricola

Source Name

239a Name

1 (Continued)

irrorata

Organic matrix protein, solubie

P. duplicatus

Source

Thais

247 Name Source

248 Name Source

249 Name Source

250 Name Source

251 Name 252 Name Source

duplicatus

Organic matrix protein, soluble

Source Name

246

Source

Organic matrix protein, insoluble Polinices

245

Source Name Source Name Source

Organic matrix protein, insoluble floridana

253 Name Source

254 Name Source

240b Name

Organic matrix protein, soluble

255 Name

Source 241a Name

T. floridana

P. virens

Source 256 Name Source 257 Name Source 258 Name Source

Organic matrix protein, insoluble

259 Name

Source

241b Name Source

242a Name

Organic matrix protein, insoluble Pila

virens

Organic matrix protein, soluble

~a~&~orna

de&sum

Organic matrix protein, soluble.. C. decisum

Organic matrix protein, insoluble Nassarius

obsoletus

Organic matrix protein, soluble Nassarius

Organic matrix (recent) shell Organic matrix Planorbis supremus shell Organic matrix Pianorbis

P. supremus-revertens

shell Organic matrix Planorbis

shell

revertens

Organic matrix Planorbis

shell

oxystoma

Organic matrix Pianorbis

troch~ormis

shell Organic matrix Planorbis

planorbiformis

shell Organic matrix Pianorb~s

sulcatus

shell

Organic matrix Planorbis heimensis

tenuis-stein-

Planorbis

steinheimensis

shell Organic matrix

shell Organic matrix protein, acid extracted Rat teeth Organic matrix protein, acid extracted Rat, Lathyritic, teeth Periostracum Mollusc (Akera soluta) Periostracum Moilusc (Aplysia willoxi) Periostracum Mollusc (Dollabella scapula)

Periostracum

AMINO

ACID ANALYSES TABLE

261

Source Name Source Name Source

1 (Continued)

Mollusc(Hydanrinaphysis)

Periostracum Mollusc (Arctica Periostracum Mollusc (Corbicula

273 islandica)

274 275

consobrina) 262 263

264a 264b 265

266a

Name Source Name Source Name Source Name Source Name Source Name Source

266b Name Source 266~

Name Source

267a Name Source

267b Name 268

Source Name Source

Periostracum Mollusc (Mulinia lateralis) Periostracum Mollusc (Mythilus edulis) Periostracum Mollusc (Pitar morrhuana) Periostracum Mollusc (P. morrhuana) Periostracum Mollusc (Solemya velum) Periostracum Mollusc (Tagelus divisus) Bermuda Periostracum Mollusc (T. divisus) Nantucket Island Periostracum Mollusc (T. divisus) Long Island, N.Y. Periostracum Mollusc (Yoldia limulara) Periostracum Mollusc ( Y. limulata) Periostracum Mollusc (Nautilus

Name Source Name Source Name Source

270 271 272

Name Source Name Source Name Source Name Source

Ovomucin (a) Chicken egg white Ovomucin (b) Chicken egg white Ovomucin I Chicken egg white Ovomucin D Chicken egg white

P Component Human spleen amyloid P, substance Sheep hydrated cyst fluid Paramyosin Clam (Mercenaria mercenaria)

276 277

278 279 280

281 282 283 284

Name Source Name Source Name Source Name Source Name

Paramyosin, light Clam (M. mercenaria) Phenylalanine-ammonia lyase Potato Phosvitin Rooster plasma Phytase Aspergillus

terreus

Polysaccharide erase

depolym-

Source Name Source Name Source Name Source Name Source

Aerobacter

aerogenes

Name Source Name Source

Pyocin R sheath

Prekeratin Cow snout Procollagen-C Chick tendon Protocollagen-C Chick tendon Protocollagen White shrimp (Penaeus setiferus)

285 286

pompilius) 269

481

XI

Number

Number

260

OF PROTEINS

287

Name Source

288

Name Source

Pseudomonas

aeruginosa

Pyocin S, Pseudomonas

aeruginosa,

strain M 47 lysate Pyrophosphatases, inorganic Bacillus subtilis, strain B 133, vegetative cell Pyrophosphatases, inorganic B. subtilis, strain B 133, spore

32 20

89

88 50

26

40

65 51

100

Leucine

lsoleucine

Protine

Serine

Threonine

308

21

tP

91

118

Methionine

Lysine

Amide

11111123

-----_

Tyrosine Tryptophan

Reference

5

20

-----_

Phenylalanine

ammonia

7

24

21

Arginine Histidine

7t13

10

acid

Glutamic Half-cystine

-1

9

acid

Aspartic

79

61

21 13

26

54

33 273

2

Valine

1”

114 121

acid

Alanine

Glycine

Amino

12

14

8

47

249

-1

83

54 34

48

67

47 27

48

61 207

3

4

29

22

29

90

97

tP

89

34 67

68

34

103 34

62

111 134

4

29

20

29

89

97

7

t13

78

36 61

58

38

106 36

59

115 134

5

37

17

27

80

139

16

-1

84

60 51

86

32

62 59

65

70 121

6

-3

22

43

32

9

49

57

0

27

107

56 107

74.

50

95 34

82

99 57

7

-

0

42.7

8.8

8.3

73.6

45.6

7.2

50.3

116.3

99.3 165.8

66.8

63.9

60.1 51.1

74.0

193 46.8

8

COMPOSITIONAL

4

-

-

36.5

32.6

20.2

31.7

67.8

5.4

5.8

102.9

81.7 81.2

138.4

739’

75.8 21.1

83.8

75.1 56.1

9

DATA:

45

-

37.5

34.6

11.9

30.7

57.3

7.2

2

102.1

87.0 83.5

124

75.74

84.5 23.6

87.8

73.4 57.2

10

Residues 111%

6

14.58

5.5

21.4

76.3

33.3

23.3

84.1

21.4

0

129.2

56.6 97.5

35.2

32.6

99.0 38.9

47.4

6

12.6’

8.0

23.6

55.8

18.0

34.9

53.0

22.8

0

102.7

46.5 79.7

39.7

22.0

90.5 33.8

36.5

13.4 32.8

12’59

6

14.48

16.9

40.1

50.0

22.0

51.8

56.3

20.0

16.9

119.7

46.4 95.0

48.7

36.9

74.0 39.1

58.6

29.0 33.7

13160

1000 residues

PER 1000 RESIDUES per

23.8 35.5

TABLE 2 RESIDUES

7

12.19

19.9

31.3

57.2

11.2

47.0

66.5

21.7

9.3

117.0

37.8 86.0

43.8

32.4

72.3 35.7

43.2

17.5 35.3

14161

7

24.9”’

13.1

26.4

41.4

15.9

27.2

44.3

17.9

5.5

76.4

58.8 59.2

43.4

35.3

70.9 22.6

35.9

9.3 24.9

1p2

7

12.3’r

13.0

47.5

46.3

28.5

38.6

57.5

16.3

15.2

122.9

53.6 70.8

55.1

39.6

77.7 31.6

69.3

27.5 35.0

16rG3

10

-13

-12 9

47 -

58

42 -

60

5

103

127

99

45

5.2

90

24

72

34

63 42

18

53 -

39

21

42

63

58

81

84

139

63

41

94

62 38

34

38

48

17

II

104

42 -14

28

6

74

34

5

71

141

80

94 47

40

% 33

63

88 63

19

E; s 7: 3 i?

141

77

12

18 -15

15

7

63

7

191 -

is

67

5

28 130

107 24

El

55 34

34

65

20

Serine Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Atginine Histidine Phenylalanine Tyrosine Tryptophan Amide ammonia Reference

Proline

Glycine Alanine Valine Leucine Isoleucine

Amino acid

52.1 64.7 79.7 70.4 65.6 31.8 32.2 66.0 92.1 132.2 8.0 85.2 46.4 33.2 39.3 27.4 51.4 13

21”

69 77 81 75 38 89 89 90 104 65 22 7 42 29 19 41 52 14

22

64 92 81 66 49 53 94 100 104 66 20 8 32 45 25 43 57 _ 14

23

75.1 82.6 60.1 84.9 43.5 54.1 78.2 68.0 98.9 117.8 17.8 15.8 62.8 40.3 21.3 35.2 34.8 8.7 _ 15

24

91 6.5 50 102 32 21 121 61 93 177 5 75 56 11 15 16 11 I_ 16

25 32 54 3.5 104 48 12 126 45 90 159 0 ‘15 154 54 0 48 0 24 17

26

28 34 126 98 55 0 33 130 81 99 130 0 0 82 34 0 48 17 _ 17

29 30 85 56 % 13 43 116 102 69 176 0 26 70 14 0 26 54 23 17

30 40 124 73 65 0 27 139 6.5 88 131 0 24 76 29 15 48 25 34 17

31 68 125 59 125 6 21 51 37 47 235 0 20 55 104 11 17 17 --I’ 18

32

Residues per 1000 residues

2 (Continued)

58 36 108 128 68 66 109 87 13 9 27 36 54 86 38 49 48 67 233 202 0 0 24 37 48 67 106 55 13 16 14 25 14 24 28 -16 17 17

27

TABLE

34

60 105 111 83 70 49 112 82 13 38 28 48 56 91 39 57 49 99 240 134 0 9 2.5 16 49 64 109 34 13 17 14 38 14 26 trz -18 18 19

33 55 91 56 113 34 37 68 54 76 178 6 23 64 69 14 33 23 tiz 19

35 52 73 52 118 52 40 82 63 97 146 20 75 39 18 33 27 ti3 19

36 48 79 56 119 49 38 72 60 88 154 7 20 67 52 18 41 27 tr2 19

37 52 75 55 116 51 39 78 61 91 146 21 73 45 19 41 27 tF 19

38 59 61 57 121 59 36 93 71 116 127 11 72 33 16 43 19 6 16

39

49 57 53 119 53 42 88 70 114 124 12 73 35 21 46 34 10 16

40

129

118.6

56.7

39.7

80.2

65.2

106.7

acid

acid

Isoleucine

Proline

Serine

Threonine

Aspartic

Glutamic

21

34

25

16

16

50.4

30.3

6.4 -------

15

Tryptophan Amide ammonia

Reference

30

16

10

22

16

18

23

28

58 8

89

91

20

31 -10

33

53 16

20

32 -20

28

64 20

20

45 -21

52

51 12

103

Phenylalanine Tyrosine

56 8

88

98

52 10

87

33.3 23.0

15

13

75.9

10

Arginine Histidine

14

922

29

29

58 19

84

3 20

192

85

47

66

7 41

Lysine

12

146 _

75

64

44

14 46

20

18

8= 21

-

31

22

tr2 -

35

38

27

30

63

53

11

7

86 110

85

19

3

85 195

47

23

22 -23

27

22

40

101

8

8

84 111

114

90 75

84

68

64

28 106

46

43

45 69

143

82

73

62

51

24

22.3 -24

31.6

33.3

23.4

41.8

57.3

16.9 t?

_ 25

23

31

15

31

97

14 24

117 91

54

63.8 98.9 111.6

71

41

55

91

76

96

73

53

95.8

48.5

46.6

77.5

65.3

78.5

86.8

52

1000 residues

81

54

53

60

64

50

per

140 6

75

43

49

Residues

2 (Continued)

74

41

48

4

188 -

87

44

59

2 44

136

85

52

59

47

17.1

177 -

84

49

57

8 64

149

53

77

45

46

Methionine

198 _

100

27

46

14 42

136

65

75

50

45

6.0

200 -

104

29

57

14 36

128

51

103

50

44

Half-cystine

98

38

57

23 40

125

47

104

59

43

185 -

122.0

48

55.1

Valine Leucine

52

104

61.4

42

52.1

41”

AIanine

acid

Glycine

Amino

TABLE

25

23

35

18

30

106

12 24

109 93

47

72

39

53

87

76

100

76

54

26

94.3

12.6

41.6

14.1

21.6

60.8

17.8 14.6

78.0 89.6

61.8

46.1

65.5

56.0

79.1

74.9

90.6

80.7

55

26

86.8

19.4

31.3

10.8

29.9

106.7

23.2

109.0 87.9

38.4

47.7

33.5

56.3

89.6

75.3

87.3

66.8

56

-

.27

29.5 -=

31.1

15.9

45.2

21.0

16.0

3.6

28

--

-

37

46

6

59

50

41

57 65

130.3 126.5

68

50

46

56

132

73

118

%

58

83.3

58.0

47.1

38.3

75.0

80.9

106.9

93.2

57

25

25

35

47

28

23 -26

-

116 86 -

65

81

42

91

9

88

147

94

59

3.7 290

-

44.8 25.4

24.0

48.2

47.7

24.3

14.3

121.2

81.7

57.2

70.4

47.8

50.6

123.5

68.6

80.7

65.7

60a

Glutamic

48

39

36

Arginine

Histidine

Phenylalanine

-

-

29b

Tryptophan Amide ammonia

Reference

29d

25.0 IO.9

42.9

23.9

17 -2Qa

Tyrosine

43.9

58

44.8

9.3 20.4

8 21

122.0

79.1

51.5

74.5

51.6

Methionine Lysine

Half-cystine

acid

Aspartic

124

64

94

acid

Threonine

90

Se&e

54.9

37

Leucine

44

56 111

Valine

Proline

44.3 122.3

85

Alanine

Isoleucine

67.9

78

Glycine

77.3

6Oc

acid

6Ob”

Amino

30

-27

-

26

37

27

5.5

59

7 20

156

99

49

58

42

43

59 119

84

60

61

31

25.3 -

25.4 43.6

49.5

13.8 54.5

-

117.0

84.0

57.5

87.8

50.0

53.3

116.0

72.0

80.7

66.5

62

32

24.0 PQ

27.0 50.0

51.0

21.0 50.0

5.0

139.0

83.0

52.0

63.0

56.0

48.0

116.0

64.0

81.0

68.0

63

33

21 -

21 35

51

19 58

12

162

97

57

72

45

41

98

65

73

63

64

34a

27.9 -2 -

31.4 44.1

48.5

17.0 52.6

10.0

127.5

89.9

57.8

75.2

50.6

34.5

114.6

62.6

90.7

70.3

65

24.2 -

39.3 53.8

39.8

15.5 60.2

9.8

110.6

94.4

51.6

76.6

39.1

31.9

69.4 114.7

97.3

71.9

46

34b

34a

96.0

60.2

85.1

65.9

42.5

68.7 93.1

72.0

74.7

66a

per

34b

24.5 -J-

18.5 41.9

46.0

17.1 56.3

-

120.4

Residues

2 (Continued)

--_c_--T.....----

22 -zQa

21 36

49

14 51

136 -

%

55

80

61

43

58 116

76

61

6%

TABLE

46 24

90

85

35

12 -30

-

-

120

91

64

66

67

51

75 83

83

79

67

38

26.3 -31

21.8 46.4

48.6

14.0 70.1

7.7

115

92.4

55.7

95.1

51.5

45.0

57.0 95.3

73.1

95.1

68a

1000 residues

38

27.9 -32

21.8 40.9

50.4

16.5 75.5

10.7

113

97.2

56.7

80.7

51.0

44.4

60.1 98.4

76.0

79.0

68b

-3 39

18

16 28

47

14 2.5

22

95

67

36

52

73

29

38 59

65

224

69

40

19.3 -34

16.1 30.3

49.2

17.6 28.6

20.7

96.3

71.8

38.7

47.5

60.9

34.8

40.5 78.9

65.9

194.0

70

40

11.0 -35

7.7 20.7

45.6

10.8 23.7

7.8

81.5

56.9

24.8

39.6

91.4

22.7

31.9 44.3

89.1

280.9

71

41

14.7 -36

16.9 30.1

41.4

14.6 14.6

17.2

86.7

57.7

32.5

45.7

79.0

33.7

36.6 66.6

53.1

225.4

72

42

15.3 -3

14.2 24.9

38.0

13.2 19.5

22.8

98.3

67.7

36.7

48.9

57.9

31.3

35.6 65.3

59.8

221

73

64.9 43

2.038

15.4

15.4 26.3

47.1

12.7 28.8

21.0

86.7

64.6

34.1

50.8

79.4

28.3

37.4 54.8

68.9

220.9

74

44a

-39

20.5

28.3

48.3 18.7

7.0 26.4

22.0

101.3

70.0

40.3

64.1 54.2

28.6

36.0 60.3

58.6

225.2

75

-

29.5

66.0

54.5

41.5

72.0

98.0

20.0

7.5 27.0

50.5 18.5

28.0

23.0

-41

28.1

69.8

49.0

40.5

70.0

97.0

22.7

5.0 26.0

48.2 14.4

26.8

22.0

-40

44a

38.0 59.0

36.0 60.2

45

60.0

65.0

-__-_---

220.0

229.0

Glycine Alanine Valine Leucine Isoleucine Proline Serine Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Arginine Histidine Phenylalanine Tyrosine Tryptophan Amide ammonia Reference

77

76'

Amino acid

44a

-42

17.0

19.0

44b

-4%

20

29

50 20

11.0

40.0

52.6 21.0

-

26

-

101

100.0

20.4

74

43

57

68

31

37 70

60

207

78a

70.0

45.5

62.8

62.0

30.0

43.0 60.0

67.0

200.0

78

44b

-42b

22

30

46 17

36

-

-

106

79

54

66

83

27

42 70

58

170

78b

46

-43

16.8

27.9

50.8 17.4

26.6

13.8

22.0

91.6

65.6

40.7

51.8

77.1

30.0

36.6 57.3

65.2

212.4

79

47

-44

32.8

45.9

55.7 26.2

59.0

124.6 22.9

91.8

52.5

72.1

59.0

39.3

62.3 104.9

75.4

75.4

80

TABLE

48

-45

24

33

56 28

73

131 11 15

95

54

85

50

46

58 87

83

78

81

31.4 49

22.4 49

-

39.5

57.6

41.9

20.1 49

-

41.2

64.2

39.5

65.8

7.9

68.6

7.6

28.6 62.6

107.7

35.4

58.9

106.0 76.9

58.0

70.4

61

39

71

69

24

39 28

62

80

84

76

50

120

35

17

34 17

68

85

85

95

13

90

85

3

17 4

93

67

86

50

41

115

54

9

24 4

89

%

87

97.2

53.4

67.6

39.6

38.8

57.4 68.0

91.0

211.9

88

50

-46

45.4

53.2

12.0

25.7

61.5

20.0

17

16

16

-

51

7

8

30

28

-

_ 52

0

8

4

8

-

51

0

0

17

45

51

-4'7

-

-

-

28

129 127 100 104

4

6.9

54a

-

68.0

20.9

11.7

23.6

64.3

14.0

87.5 59 60 38 40 123.0 1.8 262 290 316 332 -

121.5

72.9

68.2

33.2

44.1 28.6

69.5 97.2

85.6

74.4

83

72.8 119.2

56.0

45.0

82~

119.7 124.8 -

92.2

31.2

62.1

26.5

42.3

67.3 108.2

53.2

45.2

82b

68.1

87.4

98.7 29.1

86.6

27.8

85.6

37.4

28.7

49.5 112.2

55.3

52.8

82a

Residues per 1000 residues

2 (Continued)

54b

-

_

20.8

40.8

23.6

40.0

60.4

13.7

131.9 13.1

104.5

56.5

56.5

44.8

56.4

76.5 108.0

88.8

72.2

88a

103

38

77

29

1

27 30

18

153

90

32

55

38

25

26

29

41

-

-

-

-

55

24

38

17

26

34

36

121 132 177 219

125

38

81

27

6

38 29

26

142

89

3

ti

Is 3

g

F F! g

G

5

El

Reference

Glycine Alanine Valine Leucine Isoleucine Proline Serine Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Arginine Histidine Phenylalanine Tyrosine Tryptophan Amideammonia

Amino acid

92

88.0 197 83.2 145 65.0 66 71.7 11 39.9 29 56.1 54 66.9 233 66.2 136 116.9 19 120.8 57 4.6 0 14.2 0 58.4 10 47.3 27 23.4 4 38.4 0 23.5 6 4% _ _ 57 56

91a

184 131 72 16 34 54 212 124 27 63 8 2 14 27 5 9 20 -_-__ 57

93

218 100 50 43 21 90 110 128 40 62 18 5 20 50 13 26 12 -

58

58

95

196 149 75 12 30 77 199 128 22 67 11 2 12 3 3 6 6 -

94

97

177 122 67 4 16 111 201 153 21 58 tiL 1 6 43 2 4 tr 59 58

255 113 49 38 9 95 110 140 24 54 12 2 10 50 12 22 6 -49

% 99 51 54 47 40 59 115 115 133 83 50 8 46 34 12 25 32 58

98 198 136 60 52 13 % 175 148 17 53 0 0 3 36 0 16 tr -58

99

TABLE

86.9 86.3 53.8 64.3 25.7 76.2 160.1 124.6 83.2 86.1 ti2 41.9 34.9 30.0 31.4 14.4 62

105

158 68 147 86 85 71.8 113 95 106 115 99 93.8 55.6 71 86 63 111 79 45 41 56 36 38 65.6 29 16 21 28 43 20.3 97 99 119 135 80 84.8 143 113 101 157 177 131.0 117 241 95 183 181 195.6 46 47 66 32 66 61.1 65 74.3 62 70 101 62 27 23 tP 14.7 0 14 tiL tr* 20 18 37 17 33 40.1 38 34 40 14 16 25.6 4 14 13 10 12 22.6 24 14 20 8 16 25.0 8 11 1s 4 11 17.9 -50 -5% -58 58 58 60 61 61 62

110 104 67 57 31 64 148 126 73 84 0 0 38 37 11 36 14 -

104

107

100 101 102 103

106

Residues per 1000 residues

2 (Continued)

109

110

88.3 73.9 69.2 49.7 98.1 104.9 45.3 41.0 39.4 27.3 49.1 47.3 32.8 21.6 23.6 146.1 95.8 90.0 117.6 162.2 133.7 319.3 270.1 335.8 41.3 29.9 20.5 52.2 45.7 38.1 7.1 20.6 24.2 27.0 11.7 28.7 29.7 16.7 26.8 29.4 11.2 22.1 11.4 12.6 10.6 62 63 63

108

%.6 90.9 60.0 73.5 32.7 68.9 113.5 125.3 77.7 93.2 8.6 41.8 40.1 20.5 31.0 25.3 63

111

Fi

% 5

g 2 s m

t? $ 2 g

b 6

$ o

109.7

139.2

90.2

acid

acid

Setine

Threonine

Aspartic

Glutamic

10.1

11.0

52.0

49.8 8.6 %.6

64

Phenylalanine

Tyrosine

Tryptophan Amide ammonia

Reference

33

Lysine

Histidine

24.1

Methionine

Arginine

4.3

Half-cystine

74.6

39.7

45.1

57.0

Leucine

Proline

66.6

Valine

Isoleucine

92.0

105.1

112a

Alanine

acid

Glycine

Amino

64

38.2 115.5

36.5

11.2

12.0

36

12.2

-

79.0

91.5

155.0

140.5

45.5

36.3

47.3

57.1

99.3

101.3

113

_

65

27 12

66

31

46

60

14

20

73

56

65

89

41

44

95

61

9.5

106

114

65

30 17 -

70

27

38

56

14

17

77

57

67

74

41

47

100

70

99

100

115

-

65

27 15

59

31

49

61

13

17

78

58

63

92

45

42

94

57

98

102

116

-

65

26 14

60

31

49

61

13

21

76

59

63

88

43

39

91

54

99

106

117

65

23 10

50

31

60

63

15

18

97

70

54

98

44

39

89

50

95

97

118

66

28

66

29

45

60

17

16

77

58

66

101

42

45

93

62

93

103

119

TABLE

66

27

64

29

43

64

16

12

82

60

69

103

42

43

94

64

92

98

120

_ 66

29

60

24

38

57

11

10

79

61

71

110

39

45

94

65

%

106

121

Residues

2 (Continued)

66

_

29

62

27

41

60

14

15

84

65

67

101

43

45

95

65

93

97

122.

per

82

67a

-

22

49

60

100

-

-

66

71

43

%

69

15

70

23

91

142

123

45

54

90

81

67a

22 -52 _

-

-

76

76

46

95

78

22

61

20

90

146

124

1000 residues

67b

_

23

48

59

110

78

12

0

66

67

40

95

76

16

57

15

87

149

124a

68

27 -53 -

36

23

46

86

17

0

103

83

57

68

34

54

89

97

91

103

125

68

17 -54 _

29

24

22

67

2

0

93

73

42

57

25

34

53

96

70

82

126

68

13 -55 -

50

50

26

122

10

0

147

86

40

60

40

37

93

79

89

62

127

68

26 -56 -

44

34

34

92

15

13

103

107

54

54

43

39

99

72

95

76

128

68

18 -57

56

47

26

87

15

7

88

102

48

59

34

15

115

87

119

76

129

68

15 -53

44

89

68

32 -53

33

27

20

8 165

21 121 14

0

122

88

42 20 80

54

34 65

66

135

131

0

109

81

21 38 34

33

59 116

117

87

130

5 $

z

is

7;: s g

z

E-

8

5

acid

Glutamic

7.6 31.5

-

Tyrosine

Amide

Reference

-

-

Tryptophan

69

13

Phenylalanine

ammonia

56.8 13.9 -

62

Histidine

70

-

42.5 17.1

103

116.1

143.0

151

23

52.5 101.0

74.2 17.3

49 141

46 36

31 9

Arginine

Lysine

Methionine

Half-cystine

acid

Aspartic

Threonine

Serine

Prolirie

Isoleucine

Leucine

69.4 53.0

97.3 45.4

Alanine

66 44

61.0

80

98 49

Glycine

Valine

133

acid

132”

Amino

70

-

85.0 12.7 -

41.5 16.4

114.0

3.5 31.1

141.0

54.0 133.0

53.0 20.0

56.0 37.0

81.0 58.8

62.0

134

71

124.8

31.8 18.6 3.7

49.8 16.4

126.8

9.4 24.0

172.2

51.1 102.5

30.2 40.5

101.1 46.8

86.4 43.4

45.3

135

71

142.9

33.0 19.2 3.8

54.7 17.8

124.7

6.7 24.6

177.1

55.2 94.9

23.5 43.5

101.3 48.6

79.4 46.5

45.5

136

71

175.0

34.9 19.2 3.7

53.0 21.4

99.5

7.8 23.6

191.1

51.0 98.1

23.1 46.9

93.5 50.2

90.9 50.6

51.3

137

72

-

18 -

36.5

55 19

104.5

13 20

195

49.5 102

25 46

105 49

87 43

43

138

72

-

17 -

33

62 17

104.5

28

205

50 99

19 44

109 40

86 44.5

39

139

TABLE

72

-

26.5 -

34.6

59.2 28.2

76.5

14.2

115.3

55.9 105.1

51.9 69.5

83.4 48.6

82.7 63.6

86.0

140

Residues

72

-

29.3 -

32.2

46.3 32.1

73.7

14.2

110.9

46.3 128.6

49.1 82.3

84.0 21.8

98.3 33.1

119.1

141

per

2 (Continued)

8.6

73a

-

20.1 -5s

27.6

61.2 19.6

104.3

-

130.9

53.7 100.0

21.3 78.6

88.9 39.0

89.4 59.7

94.7

142a

73e

-

32.6 -

39.6

58.9 25.2

60.4

19.2

121.6

38.1 102.8

64.9 43.8

67.9 41.3

67.3 61.0

152.8

142b

1000 residues

73c

-

32.3 -

36.4

70.8 24.1

67.2

20.0

122.6

49.1 95.5

51.4 53.3

89.0 52.2

75.5 63.1

96.2

142~

-

-3 74

75

29

39

19 11

35

76 510

70

66 125

19 167

14 50

32 46

191

144

21--

20

30

207 16

61

71 135

92

46 97

38 36

66 34

83 61

69

143

75

68 -60

26

8 8

55

0.2

44

40 74

151 52

69 24

158 65

131

145

7s

12 -61

28 0.4 54

2

9 4

49

14 83

1108

25 15

231 16

338

146

171

57 130

71 144

43 25

66 39

103

148

31 65 4

-75

15

212774 60 25 -32 -a

22 49

38

2831 10 10

73

43 116

107 98

51 20

106 40

120

147

75

11 -@

9 0.2

39

4 2

29

16 47

50 2.5

110 64

30 78

411

149

75

20 10 -65

8 IO

26

24 24

113

62 150

63 85

92 47

102 61

105

1.50

s

-

3 x

;;:

-CI ;d 0

0

5 g

ti

b Ej

iz 2o

128

126 2

68

116

38

125

121 4

Isoleucine

Proline Setine

Threonine

Aspartic Glutamic

75

-

ammonia

Amide

Reference

16

-a

Tyrosine Tryptophan

1

Histidine

35

21 23

Lysine Arginine

Phenylalanine

12

Methionine

Half-cystine

90

42

Leucine

acid acid

49

69 81

-

9

75

-67

22

4

29 74

15

56

46

85

76

82

Valine

107

117

100

152

151”

Glycine

acid

Alanine

Amino

-

75

-68

14

19

6

26 25

19

114 15

132

57

88

90

36

75

40

81

159

153

-

-69

75

34 12

1

24 64

6

9

64

147

20

116

37

37 15

35

215

169

154

-

75

7 12 -70

6

43 33

12

21

105

171

47

123

61

38

68 77

74

102

155

-

75

20 17 -71

10

27 1

27

13

97

119

50

85

167

33

59 53

76

144

156

-

-

-

75

14 1

13 14

3

26

119

152

62

88

97

38

54 56

61

201

157

_ 75

18 3 -72

6

41 42

15

28

97

139

83

77

36

72 52

72

82

135

158

TABLE

-

15

17 4

2

21 21

33

16

110

136

68

95

52

75 43

66

82

159

159

_ 75

33 9 -73

4

15 38

11

14

129

113

94

72

70

123 29

46

100

101

160

Residues

15

32 -74

30

3

36 68

16

67 8

273

42

76

67

30 19

24

69

141

161

per

2 (Continued)

75

13 -75

22

27

62 48

23

109 11

85

113 62

60

79 32

42

114

98

162

15

41 19 -76

15

16 7

21

102 6

119

78 59

111

81 39

60

104

122

163

1000 residues

15

31 22 17

19

37 39

23

99 18

132

86 63

91

72 26

54

86

96

164

15

51 -78

75

-79

33

30

89 7 14

29

24

6

0.4

59

164

110 22

41

26 15

25

144

200

166

107 5

10

%

63

101

97 54

95

15 24

54

46

141

165

75

42 -80

17

15 1

25

3

3

69

143

74 23

39

50 15

38

62

380

167

_ 75

12 -81

36

5

41

42

30

4

121

113

61

93

68

31

56 89

90

109

168

75

14 -82

33

6

25

33

30

14

114

142

72

63

53

36

83

62

114

108

169

-

-= 75

33 4

5

35

34

11

11

109

141

57

83

21

41

77

61

120

158

170

-

75

-

62 -84

34

2

22 12

Is

33 1 0.4

i

3

5 7: E z

a’

z

g

25 10 104

55

5

35

64

187

348

171

30 11

94

50

13

159

38

97

acid

acid

Leucine

Isoleucine Probe

Set-me

Threonine

Aspartic

Glutamic Half-cystine

8

Reference

Tryptophan Amide ammonia

-

75

-

22 -89

12

~enylal~ine

-85

6

Histidine

Tyrosine

35

27

At&nine

75

83 37

64

21

Lysine

24

9

103 3

119

45

81

43

Methionine

61 15

58

38

81

Valine

126

190

200

173

172”

Glycine

acid

Alanine

Amino

-

75

23 -87

44

67 16

36

26

114 2

103

50

70

23 85

68

48

108

116

174

-

75

30 -88

72

57 7

15

-

7.5

-89

19

28

13 8

20

20

15

1

103

0.7

116

63

66

62

55

101

71

81

160

176

42

80

12

47

55

6

52

40

142

335

175

-

75

13 -3

40

17 0.6

11

7

3

117

133

58

91

69

40

63

60

143

91

177

65

75

-----

-

56

48 35

82

10

21

91

99

56

61

59

37

56

61

88

114

I78

6

75

-pl

12

14 2

21

12

18

94

90

47

141

61

19

69

40

176

179

179

TABLE

7

75

-91

14

11 7

39

13

17

86

117

48

121

55

25

74

42

161

163

180

2

75

41

13

10 6

37

10

8

105

125

63

145

22

35

71

60

141

145

181

Residues

75

10 -70

20

-

-3 75

7

15

8 4

17

17

16

84

110

44

103

93

25

71

42

163 181

183

75

15 -93

74

45 1

34

30

103 16

133

46

58

82

35

76

63

116 74

184

1000 residues

14 2

23

28

20

91

100

47

119

92

26

74

39

89

203

182

per

2 (Continued)

75

-

28 -3

16

6 0.2

12

1

9 4

11

11

37

57

50

121

83

19

526

185

-

11

75

-95

13

53 14

30

12

104 8

120

67

104

95

23

70

34

150 92

186

-

6

75

-M

17

2

14

15

59

21

105

117

58

106

113

17

100

26

140 89

187

75

11 -97

17

18 3

27

24

113 23

143

59

97

130

30

80

36

71

118

188

-

5

75

-98

36

44 4

39

20

12

111

117

61

65

53

44

%

62

108

121

189

75

46 -99

95

9

6

15

11

26 8

48

11

34

54

26

59

17

519 16

190

75

0.5 -1QQ

6

2

4

28

4

10

53

308

17

213

11

11

22

19

227 63

191

75

9 0.4 -101

1

2

13

19

10

62

286

21

207

25

15

27

16

64

222

192

Glycine Alanine Valine Leucine Isoleucine Proline Serine Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Arginine Histidine Phenylalanine Tyrosine Tryptophan Amide ammonia Reference

Amino acid

279 54 40 32 23 78 34 24 142 32 9 14 36 92 15 41 55 75

151 61 51 53 39 12 52 40 122 86 16 29 66 94 14 35 19 _ 75

156 64 60 61 43 71 67 43 153 96 41 8 34 39 10 40 14 -102 75

193” 194 195

1.52 70 41 49 36 64 72 49 108 85 43 21 51 72 23 44 20 _ 75

1%

198

199 207 55 62 34 32 112 54 59 120 84 104 29 16 7 2 20 5 _ _ 75

204

205

206

207

208

209

210

211 212

213

165 370 378 530 482 355 136 353 284 305 274 323 60 50 158 36 43 64 61 47 79 58 51 65 61 26 19 28 32 47 33 37 41 34 37 50 36 37 36 33 27 24 41 33 41 32 31 37 31 20 22 17 18 15 23 22 24 18 17 24 114 27 22 41 49 74 119 64 56 73 79 69 60 85 97 41 48 49 73 41 48 48 48 35 60 29 10 21 26 38 51 25 29 30 32 22 127 143 34 48 60 117 148 152 177 211 246 160 90 67 27 37 46 49 72 41 56 44 43 32 101 26 2 23 17 28 21 22 15 16 22 38 14 23 62 77 11 13 34 32 21 26 23 24 43 17 13 4 41 87 19 26 21 19 22 11 106 59 24 4 53 31 23 30 32 27 11 1 1 1 0.2 0.3 5 1 16 11 7 6 9 14 54 53 24 32 26 36 26 34 27 24 45 7 2 39 14 44 15 47 46 11 24 24 54 ~107 -108 -109 -110 -111 -112 -113 -114 -115 _ _ -116 _ _ 75 75 75 75 75 75 75 75 75 75 75 75

203

Residues per 1000 residues

2 (Continued)

200 201 202

138 143 248 318 72 98 60 46 42 39 31 17 57 36 32 19 39 25 14 5 70 156 84 43 124 82 81 1% 46 75 35 12 141 114 215 190 116 89 62 34 26 2 10 10 19 17 12 4 18 26 30 27 32 20 13 23 7 7 7 9 40 38 20 13 10 35 47 36 -tm -104 -105 -106 _ 75 75 75 75

197

TABLE

338 57 43 31 23 54 47 24 201 37 12 42 11 11 5 37 29 _ 75

214

40

31

129

40

35

36

70

38

21

198

38

10

23

Serine

Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Arginine Histidine Phenylalanine Tyrosine

Reference

-

25

21

ammonia

38

32

Amide

45

36

-

58

62

Tryptophan

303

301

Glycine

Alanine Valine Leucine Isoleucine Proline

23

41

7.5

36

3

70

-

7.5

-117

36

58 9

14

23

58

216

21Y

Amino acid

11

27

52 14

27

16

18

44

252

25

69

53

22

33

25

56

256

218

-

75

-

75

-..m1IH -119

4

25

62 19

45

35

22

53

214

39

55

59

19

30

19

48

252

217

7s

_

-120

61

36

1

34

19

38

8

37

173

IS

40

60

15

27

29

45

367

219

-

19

75

_

35

33

59

32

16

4s

46

131

32

91

37

43

5.5

58

59

211

220

-

75

-121

20

17

5

24

18

6

11

38

112

16

98

14

16

48

27

241

289

221

28

-

75

-122

20

17

5

25

18

6

11

41

118

7.5

-

-123

0.3

28

7

43

14

19

5

42

76

126 15

15 98 17

15

38

16

171

352

223

18

48

29

216

291

222

TABLE

-

75

-124

48 4

27 11

19

13 20

36

69

16

73

75

-

-125

44 0.3

7 0.3

7

3 9

38

84

23

65

46

13

25

33

12

27

127

475

225

33

20

120

453

224

4 -

75

_

75

-127

10 -126

36

30 4

20

12 18

36

72

17

63

28

8

33

17

144

461

227

45

40 10

22

9 17

44

69

20

58

38

12

39

23

97

454

226

-

75

-12R

88

69

46 8

22

1 23

3.5

41

20

60

6

20

24

14

46

475

228

Residues per 1000 residues

2 (Conrimed)

-

75

-12Y

72

40

28 1

16

14 57

73

86

37

51

58

37

47

44

54

283

229

_

2

75

-13"

24

89 19

56

21 7

62

252

43

67

80

21

33

35

63

125

230

-

4

75

-131

33

49 4

33

19 3

52

321

35

51

-

3

75

-132

28

51 5

35

20 3

52

327

45

52

55

18 23

21 25 58

39

37

206

232

36

39

217

231

-

75

-133

27

37

36 2

30

8 13

85

224

45

89

81

44 30

32

86

132

233

-

75

-134

27

27

9 3

26

6 21

70

93

75

__

-135

18

31

3

26

14

7 17

77

88

111 35 101

98

27 12

30

61

341

235

102 33

36 14

29

58

349

234

75

_

-136

20

31

12

60

19

13 II

63

66 48 194

93

31 14

51

56

214

236

Glycine Alanine Valine Leucine Isoleucine Proline Serine Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Arginine Histidine Phenylalanine Tyrosine Tryptophan Amide ammonia Reference

Amino acid

Residues per 1000 residues

2 (Continued)

211 58 51 32 12 100 62 4.5 197 64 13 10 18 61 11 32 20 -131 1.5 136 2 81 29 27 5 22 5

76

76

22 48 89 100 110

115 86 43 79

I19 6 30 34 40 8 33 20

135 84 53 97

113 125 67 135 24 2 64 29 29 57 18 23 19 26 28 23 33 29 6 I1 4 4 38 32 27 14 26 12 17 2 -------------_-____-__I-------------_---_-_I76 76 76 76

30 92 89 53 88

152 107 44 62

32 67 82 63 100

26 76 80 69 127

34 62 80 63 121

195 89 45 78

27 115 101 54 99

115 107 50 70

140 80 53 79

-

76

24 28 32 4 35 47

98

46 62 67 50 %

222 66 38 83

76

135 93 18 12 15 21 2

20 91 94 45 117

153 85 38 55

76

94 6 17 33 37 17 41 35

54 49 60 43 95

216 63 54 88

76

116 % 28 38 18 20 10

22 38 88 60 106

183 76 34 61

76

53 32 30 18 7 77 76

41 61 35 29 57

341 60 40 44

76

116 42 24 22 8 19 12

26 72 81 59 87

77

77

86 103 20 10 10 10 79 34 46 6 33 42 56 28 65 52

29 86 80 21 75

39 58 57 22 75

16 66 77 20 %

41 25 77 33 89

20 25 58 24 86

33 59 64 31 60

31 45 34 16 44

77

77

77

77

77

77

77

77

97 124 136 104 109 157 145 150 17 17 22 11 26 34 44 16 22 17 11 12 29 12 21 18 36 61 65 37 42 35 59 58 7 3 29 22 0 11 6 4 28 29 22 24 21 33 40 42 39 32 32 18 44 24 46 32 22 28 17 9 17 19 16 25

36 29 29 51 90 79 60 62 70 55 44 32 95 118 108

182 111 105 122 126 147 181 130 187 120 170 123 85 101 121 93 108 157 115 155 89 161 51 59 56 59 57 49 39 58 41 51 57 78 53 40 52 47 61 33 58 36 50 45

231a 238a 238b 239a 239b 240a 240b 241a 241b 242a 242b 243a 243b 244 245 246 247 248 249 250 251 252 253

TABLE

iz .3 5 $

F U ir 3” B jq

8z

Glycine Alanine Valine Leucine Isoieucine Proline Serine Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Arginine Histidine Phenylalanine Tyrosine Tryptophan Amide ammonia Reference

Amino acid

255

256

257

127 177 74 91 104 74.3 101 89 41.9 43.9 73 71 52.7 39.5 90 93 31.8 28.3 57 48 84.0 85.1 4.5 37 81.3 80.9 112 120 53.0 57.4 33 52 148 163 to9 118 134 161 130 141 4.7 6.4 2 2 5.7 7 5 15.0 50.1 31.5 19 23 36.0 31.1 39 21 49.0 29.6 1 20.4 18.8 40 31 12.5 8.5 12 12 -138 -139 ----18 78 75 75

254a

259

72 89 66 loo 57 38 73 72 132 137 7 10 38 45 8 33 14 -140 75 75

73 79 68 104 68 31 105 55 13.5 115 1 5 35 30 42 49 8 _

258 669 21 11 12 21 25 23 5 16 13 14 20 19 33 6 13 70 -141 75

260

262 286 79 50 43 29 48 65 54 105 68 8 3 38 61 10 34 12 -144 75

264a 264b

510 333 38 61 43 49 27 42 16 29 24 47 60 54 19 47 52 91 15 58 2 10 3 3 15 37 46 59 12 9 21 34 97 30 -193 -144 75 75

263

Residues per 1000 residues

2 (Continued)

626 435 28 53 12 32 28 34 10 34 23 51 17 40 8 22 23 74 12 36 5 22 34 64 13 16 17 46 21 1 29 42 93 9 -142 75 75

261

TABLE

571 49 53 12 5 54 26 25 IO 15 7 27 14 6 I 82 44 75

547 54 57 10 4 54 28 24 7 13 7 19 24 18 4 84 39 75

266a 2661, 266~

494 550 41 42 42 34 21 16 27 8 54 37 89 20 II 23 29 23 19 24 4 0.4 72 60 13 24 13 2.5 3 3 54 41 12 69 -143 -130 75 75

265 648 17 18 6 5 34 29 9 32 9 4 17 20 38 4 85 13 75

269

661 132 64 18 46 45 16 49 63 41 58 6 5 28 46 32 74 51 26 67 76 9 48 75 35 224 100 9 101 100 4 38 79 17 3 16 22 17 61 34 35 29 4 21 86 19 39 13 12 32 3614Q . --75 75 79

267a 267b 268

Glycine Alanine Valine Leucine Isoleucine Proline Serine Threonine Aspartic acid Glutamic acid Half-cystine Methionine Lysine Arginine Histidine Phenylalanine Tyrosine Tryptophan Amide ammonia Reference

Amino acid

271

272

273

68 70 72 79.6 46 49 56 50.6 64 76 73 82.5 61 63 68 94.6 46 55 52 53.0 58 46 57 55.8 76 48 71 74.1 72 63 72 42.6 110 113 118 86.2 108 115 87 107.2 83 8.0rS3 78 70 16 18 19 3.1 56 60 40 55.4 18 43.2 34 29 20 20 13 21.2 40 41 48 49.0 37 54.1 34 36 12’50 18 16 3.7 -151 -152 _ 79 80 79 n

270”

70.8 92.3 76.2 63.8 24.7 61.7 98.5 100.8 74.0 158.3 2.6 15.5 36.0 36.2 21.5 60.0 7.0 -1% 81

274

276

22 18 122 141 43 41 121 135 34 33 50 43 42 47 131 145 211 206 15 10 74 69 101 87 6 4 8 5 21 17 80 80

275 67.5 88.8 84.1 98.5 52.0 47.6 52.3 29.7 95.4 116.0 20.6 66.8 41.8 23.1 36.4 29.7 81

36 39 21 17 9 19 536 22 67 48 0 3 75 44 53 6 5 _ 82

278 83 75 48 78 41 72 69 74 112 97 17 23 34 43 20 51 45 17 _ 83

106 78 70 66 55 34 82 75 129 84 10 8 40 52 18 46 34 13 _ 84

279 -280

282

164 326 67 127 40 23 92 28 35 I1 14 122 111 25 40 18 91 36 141 73 6 4 13 51 18 61 48 6 10 36 15 28 -1.55 _ 85 86

281

Residues per 1000 residues

2 (Continued)-

277

TABLE

39.1 15.7 26.3 30.6 148.5 89.8 9.0 13.6 64.3 42.8 7.4 49.2 43.0 190.4 -157 87

14 208 25 22 51 79 5 39 45 4 13 -15.5 86

-

53.9 72.0 49.4 55.0

284

313 119 24 30

283

75.4 8.1 12.7 40.0 53.1 9.0 37.6 20.4 22.9 _ 88

47.3 40.7 68.3 58.4 113.8

84.1 153.3 84.4 70.5

285

115.8 0 19.8 80.8 52.3 !.I.4 23.6 11.2 3.7 89

47.4 36.6 73.2 61.0 95.6

78.3 128.0 84.5 91.2

286

151 14.5 85.2 25.0 12.6 28.1 22.1 90

64.9 26.2 42.3 48.0 126

52.0 109 84.3 108

287

145 13.5 83.8 25.2 13.3 27.6 23.4 90

66.2 26.2 44.0 48.8 126

52.0 115 84.0 106

288

: tl .g z H Fi Is 5

E3

n Protein number corresponds to that given in Table 1. I Not reported (--). * tr, Trace. 3 The amino acid analyses of free secretory piece is in Ref. (2) and is similar to that of the bound material. Carbohydrate content of free secretory piece expressed as a percentage: gafactose, 3.12; mannose, 3.95; fucose, 2.26; hexosamine, 4.36; sialic acid, 1.9. 4 No hydroxyproline detected. ’ See also Ref. (5) for analysis of pooled IgG. B Carbohydrate residues, percentage: hexoses, 5.0; fucose, O.t;acetylhexosamines, 3.6; sialic acid, 3.4 ’ Carbohydrate residues, percentage: hexoses, 9.9; fucose. 0.7; acetylhexosamines, 7.4: sialic acid, 3.4. 8 Carbohydrate residues, percentage: hexoses, 3.1; fucose, 0. I; acetylhexosamines, 3.2; sialic acid, 2.0. 9 Carbohydrate residues, percentage: hexoses, 6.2: acetylhexosamines. 4.1; sialic acid, 3.1. lo Carbohydrate residues, percentage: hexoses, 10.8; fucose. 0.4; acetylhexosamines, 9.2; sialic acid, 14.3. I’ Carbohydrate residues, percentage: hexoses, 3.2; fucose. 0. I; acetylhexosamines, 2.7; sialic acid, 1.7. See also Ref. (8). ‘* ~-Acetylglucosamine, 8.2%. I3 Glucosamine, 3.7%. In Ref. (IO), the amino acid analyses of the inhibitor dimer and the monomer and dimer isolated from deproteinized plasma are also given. I4 In Ref. (II), the amino acid analyses of fractions obtained by treating SCMK-A with different proteases may be found. r5 In Ref. (l2), the amino acid analyses of fractions obtained by treating a-keratose with different proteases may be found. I6 Less than 10 residues. In addition to these electrophoretic bands (Nos. 26-31). the analyses of two more DEAE functions are given in Ref. (17). I7 This result is the mean value of the analyses ot two samples from two patients. r” Analyses of VLDL subfractions are also given in Ref. (19). Is Composition of constituents corresponding to 100 mg of proteins: I .3 mg of phospholipid and 1.2 mg of hexoses. *OComposition of constituents corresponding to 100 mg of protein: 34 mg of protein, 0.9 mg of phospholipid, and 0.78 mg of hexoses. 2’ Composition of constituents corresponding to 100 mg of protein: 60 mg of protein, 0.4 mg of phospholipid. and 0.43 mg of hexoses. *2 Amino acid composition of electrofocused fractions also given in Tables 7 and 8 of Ref. (21). 23This is the analysis of preparation 310. The analyses of other preparations are given in Table 3 of Ref. (23). 24Carbohydrate composition (g/l00 g of protein): glucosamine. 2.49; total hexose, 6.00; glucose, 2.24: galactose, 2.25; mannose, 1.51: fucose, trace; sialic acid, 2.55. 25 The amino acid analysis of the outer layer is given in Table 2 of Ref. (27). 26 Amino acid analyses of a red pellet and colorless supernatant given in Table 2 of Ref. (28). 26a Amino acid analysis of lipid-extracted membrane (29c,d). 27 Amino acid analyses of the protein precipitated at pH 4.5 and of the protein soluble at pH 4.5 can be found in Table 2 of Ref. (31). 28 Amino acid analyses of water layer after butanol and pentanol extraction can be found in Ref. (32a). Additional amino acid analyses of membrane fractions can be found in Ref. (32b). *9 Glucosamine, 1.74 pmol. 29aThere are 2.1 mol% glucosamine and 0.5 mol% galactosamine. All resuhs are based on IO0 residues of amino acids plus amino sugars. 2YbThere are 1.12 mol% glucosamine and 0.50 mol% ~lactosamine. All results based on I00 residues of amino acids plus amino sugars. 3o Amino acid analyses of five fractions of fat soluble membrane from :

% s 2 ; z

b E; h sz 2 i

3

G

milk have been reported in Ref. (36) and of other fat globule membrane 3RAlso found were 81.1 residues of hydmxyproline, 21.3 residues of hydroxylysine, 15.8 residues of glucose, 18.3 residues of galactose, 4.32 proteins in (37). 31~arbohydmte composition (nmokmg of protein): fucose, 16.4; residues of mannose, 0.98 residues of fucose, 7.40 residues of N-acetyl2.67 residues of mannose. 47.8; galactose, 94.0; glucosamine, 63.6; sialic acid, 13.9: glucosamine, 0.99 residues of N-acetylgalactosamine; N-acetylneumminic acid, and 16.13 residues of glycosylgalactosylglucose, 23 1. 32 Carbohydrate composition (nmokmg of protein): fucose, 31.1; hydroxylysine. Also reported are selected analyses of the diabetic human mannose, 92.4: galactose, 189; glucosamine, 138; sialic acid, 24.2; glomerular basement membrane. glucose, 306. 3g Also found were 24.5 residues of hydroxylysine, 12.0 residues of B There were also found three residues of 3-hydroxyproline. 67 residues 3-hydroxyproline, and 53 residues of 4-hydroxyproline. of 4-hydroxyproline, and 22 residues of hydroxylysine. Carbohydrate 4u Also found were 25.0 residues of hydroxylysine, 8.5 residues of composition (mg/lOO mg of protein): glucose, 2.6: galactose, 2.7; mannose, 3-hydroxyproline, and 56.5 residues of Chydroxyproline. 1.1; fucose, 0.45; hexosamine, 1.6; sialic acid, 1.05. Amino acid analyses *r Also found were 22.5 residues of hydroxylysine, 7.0 residues of of soluble and insoluble fractions, obtained by autoclaving at 110°C and 3-hydroxyproline, and 56.0 residues of 4-hydroxyproline. tritiated glomerular basement membranes are in Ref. (39). *P,Also found were 21.8 residues of hyd~xyfysine, 7.0 residues of 3* There were also found 8.6 residues of 3-hydmxyproline, 58.2 residues 3-hydroxyproline, and 50 residues of 4-hydroxyproline. of hydroxyproline, and 18.4 residues of hydroxylysine. Carbohydrate 42aThere are 25 residues of hydroxylysine, 26 residues of 3-hydroxycomposition (mg000 mg of protein): glucose, 1.6; galactose, 1.8; mannose, proline, and 58 residues of Ilhydroxyproline. 1.0; fucose, 0.45; hexosamine, 1.25; glucosamine, 0.90; galactosamine, 42bThere are 17 residues of hydroxylysine, 27 residues of 3-hydroxy0.29: neuraminic acids, 0.51; glycolyl, 0.38; acetyl, 0.13. proline, and 50 residues of 4-hydroxyproline. 35There were also found 3.3 residues of 3-hydroxyproline, 87.8 residues of 43 Also found were 77.1 residues of hydroxyproline, 21.8 residues of and 13.4 residues of hydroxylysine. Carbohydrate hydroxyproline, hydroxylysine, 17.1 residues of glucose, 22.1 residues of galactose, 5.5 composition (mg1100 mg of protein): giucose, 2.5; galactose, 2.9; mannose, residues of mannose, 1.0 residue of fucose, 12.0 residues of hexosamines, 1.1; fucose, 0.5: hexosamines, 1.40; glucosamine, 1.20; galactosamine, and 2.6 residues of sialic acids. In Table 2 of Ref. (46) is also found the 0.30; neuraminic acids, 1.05; glycolyl, 0.80; acetyl, 0.25. amino acid and carbohydrate analyses of urea-extracted and SDS-extracted 3sThere were also found 18.4 residues of 3-hyd~xyp~line, 84.6 residues membranes. of 4-hydroxyproline, and 29.9 residues of hydroxylysine. The amino acid 44 In Table 1 of Ref. (47) is found the amino acid analyses of 16 fractions analyses of KCI, KBr, and sodium trichloroacetate extracts are given in obtained from the nuclear membrane. Table 1 of Ref. (41). 45 Also found (g/100 g): carbohydrate, 5.81; glucose, 2.11; galactose, 37 There were also found 22.2 residues of 3-hydroxyproline, 81.3 1.51; mannose, 0.82; fucose, 0.09; N-acetylglucosamine, 0.79; N-acetylresidues of 4-hydroxyproline, and 26.1 residues of hydroxylysine. Also found were (~mol/lOO mg) sialic acids, 2.99; glucosamine, 6.04; glucose, galactosamine, 0.19; sialic acid, 0.30; lipids, 24.00; phospholipid, 20.30; cholesterol, 3.70; RNA, 0.23; DNA, 0.09. 17.2; galactose, 18.5: fucose, 1.6; mannose, 3.5; and (g/100 g) phospho46 Also found were 21.5 residues of glucosamine and 56.4 residues of lipids, 2.05; cholesterol, 0.75. E 3 3 5 z

is E; ;4: ?$

;f; 00

a Hydroxylysine, 0.3 residues, 58 &g of glucosamine, 26 pdg of galactosamine. In Table 2 of Ref. (50) is found the amino acid analyses galactosamine. of the Tween 20 supematant and the Tween 20 residue. 6JGlucosamine, 11 &g, gaiactosamine, 4 ppig. ” Kagi (53) reports that histidine and aromatic amino acids are absent. +XNine residues of hydroxyproline, 76 &g/g of glucosamine, 55 CLglg of 4* Also found were 10.9 residues of cysteic acid, and 4.7 residues of galactosamine. omithine. 67 Glucosamine, 164 &g, galactosamine, 5 &g. 4sThe following are reported as moles/l00 moles of carbohydrate: BsThree residues of hydroxylysine, 3 j&g of glucosamine, 1 &g of N-acetylneuraminic acid, 9.0; galactose, 27.6; fucose, 21.7; N-acetylgalactosamine, 22.8; N-acetylglucosamine, 18.8; uranic acid, 0: sulfate, galactosamine. 69 Hydroxylysine, 0.5 residue, 113 pg/g of glucosamine, 6 LLg/B of 12.8. SoAlso found were carbohydrates (PmoYmg): galactose, 1.19; hex- galactosamine. ‘O Glucosamine, 1 I.Lg/g. osamine, 2.17; fucose, 0.52; sialic acid, 0.02. 7* Glucosamine, 34 &g. 51 Also found were carbohydrates @mol/mg): galactose, 1.44; hex‘* Glucosamine, 16 &g, galactosamine, 5 cLp/g. osamine, 1.66; fucose, 0.82: sialic acid, 0.02. 62The amino acid analysis of a minor component is given in Table I ra Glucosamine, 5 cLg/g, galactosamine, 27 kg/g. r4 Glucosamine, 1 &g. of Ref. (67). 75Two residues of hydroxylysine, 16 @g/g of glucosamine, 3 &g of s3 Glucosamine, 0. galactosamine. 54Glucosamine, 27 mol%. 76Glucosamine, 3 &g, galacto~mine, 2 &g. sj Glucosamine, 7’mol%. 77Glucosamine, 4 pdg, galactosamine, 1 pg/g. 56Glucosamine, 50 mol%. ?BFive residues of hydroxylysine, 29,329 pg/g of glucosamine. 5’ Glucosamine, 6 mol%. 74 Two residues of dihydroxyphenylalanine, 0.4 residue of hydroxyly58 Also in Table 2 of Ref. (73a) are the amino acid analyses of the acid-extract and acid-insoluble material. Phosphoamino acids expressed as sine, 765 cLg/g of glucosamine, 102 @g/g of galactosamine. 8o One residue of dihydroxyphenylalanine, one residue of hydroxyly3.5 mol% serine. See also Ref. (73b) for amino acid analyses of sine, 420 pgis of glucosamine, 11 &g of galactosamine. fractions of Walker tumor extracts. 81 Glucosamine, 23 p&g, galactosamine, 8 &g. 5y Also found were 3 residues of hydroxylysine, 80 mg/g of glucosamine, ** Glucosamine, 89 pg/g. galactosamine, 49 I*glg. and 265 mg/g of galactosamine. 83 Glucosamine, 8 &g. B” Also found were 16 residues of hydroxylysine, 245.7 mg/g of *4 Glucosamine, 962 &g, galactosamine, 25,156 p&/g. glucosamine, and 3 mgig of galactosamine. 85 Glucosamine, 0.2 &g. ‘* Also found were 0.4 residues of hydroxylysine, 644 mg/g of 86 One residue of hydroxylysine, 0.7 @g/g of glucosamine, 0.3 &g/g of glucosamine, and 248 mg/g of galactosamine. galactosamine. ‘* Also found were 5.7 mg/g of glucosamine and 57 mg/g of galactosamine. 87 One residue of hydroxylysine, 8 CLgig of glucosamine, 4 &g of 63 Also found were 12 residues of hydroxylysine. 10.0 mgig of galactosamine. glucosamine and 12 mg/g of galactosamine. $ \o

:

32

% s

iz

E ~

b ‘3 9

0

*@Seven residues of hydroxylysine, 8 I LLglg of glucosamine, 72 cLg/g of galactosamine. ss Glucosamine, 5 j&g. so Thirty-eight residues of hydroxyproline, four residues of hydroxylysine, 46 &g of glucosamine, 6 j~g/g of galactosamine. s* Glucosamine, 2 &g. 82Glucosamine, 0.7 beg/g, galactosamine, 0.8 @g/g. W Glu~osamjne, 30 F&g, galactosamine, 5 @g/g. w Nine residues of di~yd~xyp~enyla~ine, 186 pg/g of glucosamine, 64 ~~g/g of galactosamine. s5Glucosamine, 0.7 @g/g, gala~to~mine, 0.2 LLg/g. 96 Glucosamine, 1.5@g/g, galactosamine, 2 @g/g. 97 Glucosamine, 7 &g, gafactosamine, 1 w&g. sa Glucosamine, 8 &g. galactosamine, 9 &g. 99Glucosamine, 0.3 &g. r* One residue of hydroxylysine, 4 ccglg of glucosamine, 4 @/g of galactosamine. w One residue of hydroxylysine, 4 &g of glucosamine, 3 /&g of galactosamine. lo2 Glucosamine, 11 p&g, galactosamine, 4 &g. rW Glucosamine, 14 &g, gaiactosamine, 14 p&g. lw Seven residues of hydroxylysine, 64 &g of glucosamine, 88 &g of galactosamine. *05Glucosamine, 4 @g/g, galactosamine, 1 ,r&g. lo6 Hydroxylysine, 0.3 residue, glucosamine, 11 @g/g, galactosamine, 2 ccglg. rM Glu~o~~ne, 14 &g/g, galactosamine, 1.5tlgfg. ro8One residue of hydroxylysine, 20 &g of glucosamine, 2 &g of gatactosamine. ‘09 One residue of hydroxylysine, 232 y&g of giucosamine, 21 lLglg of galactosamine. If@Seven residues of hydroxylysine, 11 Leg/g of glucosamine, 11 pg/‘g of galactosamine. rrl Glucosamine, 37 pg/g.

rr2 Glucosamine, 15 ELg/g, galactosamine, 24 &g. ‘la Glucosam~ne, 41 &g/g, galactosamine, 30 LLglg. rl* Glucosamine, 4 &g, galactosamine, 5 I.L$l&. *I5 Glucosamine, 7 @g/g, galactosamine, 9 &g. rr8 Glucosamine, 4 &g/g, gaiactosamine, 5 .ug/g. r*t Glucosamine, 20 &g, galactosamine, 25 &g. rr8 Glucosamine, 14 &g, galactosamine, 16 &g. rln Glucosamine, 25 pg/g, galactosamine, 30 LLg/g. r20 Glucosamine, 5 g&g, galactosamine, 11 f.&g. r2r One residue of dihydroxyphenylalanine, 25 pg/g of glucosamine, 16 &g/g of galactosamine. r2* Five residues of dihydroxyphenylal~ine, 75 &g of glucosamine, 47 &g/g of galactosamine. 123Two residues of dihydroxyphenylalanine, two residues of hydroxylysine, 89 &g glucosamine, 19 &g galactosamine. rz4 Hydroxylysine, 0.7 residue, glucosamine, 22 @g/g, galactosamine, 4 lack. rz5 Glucosamine, 10 @g/g. I20 Hydroxylysine, 0.6 residue, glucosamine, 73 &g, galactosamine, 1.5E”g/g. rz7 Glucosamine, 139 @gig, galactosamine, 12 j&g. I*8 Glucosamine, 215 clg/g, galactosamine, 14 &g. lx* Glucosamine, 12 ItpJg. r30 Glucosamine, 15 &g, galactosamine, 4 @g/g. r3r Galactosamine, 79 &g. Ia2 Galactosamine, 53 &/g. *33Glucosamine, 28 &g, galactosamine, 16 EL&/g. 134Glucosamine, 6 pg/g. 135Glucosamine, 5 LLg/g. r3* Three residues of hydroxylysine, 5 &g of gIucosamine, 13 &g of galactosamine. r5’ Three residues of hydroxyfysine, 5 LLg/g of glucosamine, 14 &g of galactosamine. .. _^^ __. .. “.. lJs Nineteen resulues ot hydroxyproime.

Ia9 One residue of dihydroxyphenylalanine. WI Twelve residues of hydroxyproline. I41 Nine residues of dihydroxyphenylalanine. r4* One residue of hydroxylysine. I43 Two residues of dihydroxyphenylalanine. *U Five residues of hydroxylysine. 145Seven residues of dihydroxyphenylalanine. Ia6Twelve residues of hydroxylysi~e. 14r Four residues of hydroxylysine. I** Sixty-five residues of hydroxyproline. rl@Carbohydrate (%, w/w): glucosamine, 13; galactosamine, I50 Carbohydrate (%, w/w): glucosamine, 1.9; galactosamine, I51Carbohydrate (%, w/w): ghtcosamine, 3.2: galactosamine, lose, 12.3; sialic acid, 4.6. Is2Carbohydrate (%, w/w): glucosamine, 5.6: galactosamine, 1.6.

4.6. 0.4. 0.8; man-

IS3Residues of cysteic acid listed: 36.3. 154Carbohydrates (nmoUmg): D-&CO%, 134; o-gaiactose, 780; N-acetylo-galactosamine, 310; IV-acetyl-o-glucosamine, 286; mannose, 36: fucose, 26; glucuronic acid, 124. Is5 Half-cystine residues, less than 4: tyrosine, less than 1 residue: 17 residues of hydroxylysine; 99 residues of hydroxyproline. 156Half-cystine residues, hydroxylysine, hydroxyproline, and tyrosine: ail less than 1 residue. 157No residues of hydroxylysine or hydroxyproline detected. Is8 Multiply values for No. 11 by I. 1.5. Is9 Multiply values for No. 12 by 1.38. rW Multiply values for No. 13 by 1.15. I61 Multiply values for No. 14 by 1.25. IS2Multiply values for No. 15 by 1.53. lfi3 Multiply values for No. 16 by 1.17.

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