Veterinary World, EISSN: 2231-0916 Available at www.veterinaryworld.org/Vol.9/July-2016/7.pdf

RESEARCH ARTICLE Open Access

A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay Anju Mohan1, Hari Mohan Saxena1 and Puneet Malhotra2 1. Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India; 2. Department of Animal Genetics and Breeding, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana - 141 004, Punjab, India. Corresponding author: Hari Mohan Saxena, e-mail: [email protected], AM: [email protected], PM: [email protected] Received: 07-04-2016, Accepted: 08-06-2016, Published online: 12-07-2016 doi: 10.14202/vetworld.2016.717-722 How to cite this article: Mohan A, Saxena HM, Malhotra P (2016) A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay, Veterinary World, 9(7): 717-722.

Abstract Aim: We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19. Materials and Methods: The antibody titers were measured by standard tube agglutination test (STAT), microtiter plate agglutination test (MAT), indirect hemagglutination assay (IHA), and indirect enzyme-linked immunosorbent assay (iELISA) as per standard protocols. Results: The mean STAT titers were 1.963±0.345 in infected cattle and 1.200±0.155 in healthy vaccinated cattle. The difference was extremely significant (p

A comparison of titers of anti-Brucella antibodies of naturally infected and healthy vaccinated cattle by standard tube agglutination test, microtiter plate agglutination test, indirect hemagglutination assay, and indirect enzyme-linked immunosorbent assay.

We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with str...
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