4274 Nucleic Acids Research, Vol. 18, No. 14

A cDNA clone from Pisum sativum encoding a low molecular weight heat shock protein Lisa M.Lauzon, Kenneth W.Helm* and Elizabeth Vierling Department of Biochemistry, Biological Sciences West Building, University of Arizona, Tucson, AZ 85721, USA GenBank accession

Submitted June 1, 1990

no.

cDNA represents the second complete sequence reported for this class of HSPs in plants.

Pisum sativwn synthesizes multiple low molecular weight (1mw) heat shock proteins (HSPs) when subjected to high temperature stress. We have characterized a cDNA encoding one of the lmw HSPs. A cDNA library was prepared using mRNA isolated from leaves of P. sativum (cv 'Little Marvel') which had been heat shocked for 120 min at 39°C (1). The library was screened at reduced stringency with a cDNA clone encoding a soybean (Glycine max) lmw HSP representing the class VI HSP gene family defined by Raschke et al. (2). Pshspl7.7 was identified and found to hybridize to a transcript whose synthesis was heat inducible in P. sativum plants. Pshspl7.7 encoded a 157 amino acid, 17.7 kDa protein which is 78.3% identical to Gmhspl7.9-D, as aligned below. Dashes represent identical amino acids, and asterisks indicate a gap inserted to optimize the alignment. This

ACKNOWLEDGEMENT This research 88-37264-3914.

was

supported by USDA-CRGO grant

REFERENCES 1. Vierling,E. and Sun,A. (1989) In Cherry,J.H. (ed.) Environmental Stress in Plants; Biochemical and Physiological Mechanisms. Springer Verlag, New York, pp. 343-354. 2. Raschke,E., Baumann,G. and Schoffl,F. (1988) J. Mol. Biol. 199, 549-557.

10 20 30 40 60 70 80 50 90 CAAAAATCAAAACGTGCGACAAACACAAAATCATCCCACAAAGAAAGCAATGGATTTCAGGCTAATGGATTTGGATTCTCCACTCTTCAA M D F R L M D L D S P L F N Pshspl7.7 Gmhspl7.9-D V - G - E - - - - H 100 110 120 160 170 130 150 180 140 CACTCTCCATCATATAATGGACCTCACCGACGACACAACCGAGAAGAAC******TTAAACGCTCCAACTCGAACATATGTCCGTGACGCAAAGGC T L H H I M D L T D D T T E K N * * L N A P T R T Y V R D A K A Q - M - -M S E - G A G D - K T H - - - - W S 190 200 210 270 220 250 260 230 240 AATGGCTGCAACTCCAGCGGACGTGAAAGAGCATCCAAATTCATACGTGTTTATGGTGGACATGCCTGGGGTGAAATCTGGTGACATAAA M A A T P A D V K E H P N S Y V F M V D M P G V K S G D I K Y-EI - - - - L 280 290 300 310 320 360 330 340 350 GGTTC!Ar.GTr.rAAr.ATrrA,A^TGTr.rTAvTaT,ATAA.Tr.r-rr.Ar.Ahr.r.AAr-A.r.Ar-ArA-ArAA^ArA ^Ar-rTaTTA A TATTTr-hA^rATr-rA V Q V E D E N V L L I S G E R K R E E E K E G V K Y L K M E D - L - - - CD -A - - - R - 370 380 390 400 410 420 430 440 450

AAGAAGGATTGGTAAGTTGATGAGGAAATTTGTGTTACCTGAGAATGCGAATATTGAAGCTATCTCTGCTATTTCTCAAGATGGTGTTCT R

R

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I G K L M R K F V L P E N A N I V -T

E

A

I

S

A

I

S

D

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V

C

Q

D

G

V

L

460 470 480 490 S00 510 520 530 540 TACGGTTACAGTTAATAAATTGCCTCCACCTGAACCTAAGAAACCAAAAACTATTCAAGTTAAGGTTGCTTGATCGGTGTACGATTTCAT T S

V

T

V

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550

N Q

K

L

P

P

P

E

P

K

K

P

-

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-

560

570

580

K R

T

I

Q

V

K

V

A

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-

590

600

610

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630

GTCAACAAATCAGAAGGAATGTTTGTCTTTTTAGTTGGTTTGTGTAGCAATGGTTTTGTGTGTTTTCGCCTAGTTGGCCCTATATATGAT 640 650 640 670 680 690 700 710 720 GATCATCATGCGATGTAATTTGTAACAATATGACATGAATGAATTTTAATTACTTGGTTTTTCTGCTTGTAACATTGTTGCGTTGCCCCC 730 740 750 760 770 ATGATAAAATTGAGAAACTGAAGTATTAAAGAAAAGAAAATGTTTCATTTAC *

To whom correspondence should be addressed

M33901

A cDNA clone from Pisum sativum encoding a low molecular weight heat shock protein.

4274 Nucleic Acids Research, Vol. 18, No. 14 A cDNA clone from Pisum sativum encoding a low molecular weight heat shock protein Lisa M.Lauzon, Kennet...
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